Background and objectives: Errors in blood grouping are occasionally due to anomalous antibodies or extraneous materials in the reagents. We investigated the case of a volunteer blood donor who was originally grouped as AB, Rh-positive,by slide grouping. However, her washed red cells showed group B, which agreed with her reverse serum grouping. Materials and methods: Standard serologic techniques were employed throughout. Results: Investigation revealed an unusual autoantibody in the donor’s blood that preferentially agglutinated the red cells in the presence of citrate. Because the grouping reagents contained citrate, this led to a false grouping when whole blood was tested rather than washed red cells. The antibody reacted at 37°C and at 4°C, but failed to agglutinate red cells when 0.1 M solution of monosaccharides was added to the reaction mixture. The antibody agglutinated all red cells except 6 examples of the‘Bombay’ phenotype included in the panel. The antibody was neutralized by H secretor saliva, and its activity was abolished by DTT reagent, indicating its possible IgM nature. Conclusion: These findings suggest that this anomalous antibody has anti-H specificity.

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1997
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