Objectives: Platelets prepared after holding of whole blood overnight at 22 °C have a well-preserved metabolism. However, the possibility that such prolonged incubation with active granulocytes may increase platelet activation has not been fully tested. Methods: We investigated this possibility by flow cytometric analysis of membrane glycoproteins (GPs) lb and Ilb/IIIa and the activation markers CD62P and CD63 in platelet concentrates (PCs) prepared from whole blood that was held for either 6 h without cooling plates (n = 20) or for 24 h on cooling plates of 1,4-butanediol (n = 20). PCs were prepared by the platelet-rich plasma method and analyzed on the second storage day. Results: Platelet yield and aggregation response to ristocetin, collagen and epinephrine + ADP were similar in both types of PCs, as was the mean fluorescence intensity for GPs lb and Ilb/IIIa. PCs prepared by the overnight-hold method did not differ from those obtained 6 h after collection in the percentage of platelets expressing CD62P (12.3±6.2% vs. 14.1±4.0%; p>0.1) or CD63 (9.8±6.4% vs. 8.8±3.6%; p>0.1). Conclusion:Prolonged holding of whole blood at 22 °C prior to component preparation does not increase the level of platelet activation.

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1997
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