Femoral heads (FHs) are a source of abundant hematopoietic cells. a Flow cytometry plots of cells from a representative FH (Sample 7) following cell separation with CD3, CD14, or CD34 microbeads are shown and demonstrate high purity for each cell type. b CD34⁺ cells from 5 FH samples (Sample # indicated along x-axis) and 1 cord blood (CB) sample were grown in methylcellulose with cytokines and colonies were counted after 14 days. The mean of 2 plates are shown as short black lines for each sample. Circles and squares of the same color represent the replicates for a given sample. Data from these samples were published in a normalized format in Heaton et al. [10]. c CD34⁺ cells from 1 FH and 2 CB samples were evaluated by Western blot with anti-β-actin and IRDye 800. The traditional black and white image and the fluorescent image are shown.