Fig. 2
Gating strategy in newly diagnosed AML to identify CD34+CD38-CD123+ cells [30]. a Ungated: to identify the blast population based on CD45 dim and low SS log and CD34 backgating. b Ungated: to identify CD34+ cells and the blast population on the CD45/SSC log density plot. c Gated on CD34+ cells: to identify a population homogenous for CD34+ cells. d Ungated: to identify CD34+ cells which resemble the blast populations. e Ungated: to identify a CD38+ population from the total population. f Ungated: to identify a CD123+ population from the total population. g Dot plot: gated on CD34+ homogenous cells. h Density plot: gated on CD34+ homogenous cells. i Density plot: gated on CD34+CD38- cells to identify a population homogenous for the scatter. j Dot plot: gated on CD34+CD38- homogenous cells. k Density plot: gated on CD34+CD38- homogenous cells. l Density plot: gated on CD34+CD38- homogenous cells to identify a population homogenous for the scatter.

Gating strategy in newly diagnosed AML to identify CD34+CD38-CD123+ cells [30]. a Ungated: to identify the blast population based on CD45 dim and low SS log and CD34 backgating. b Ungated: to identify CD34+ cells and the blast population on the CD45/SSC log density plot. c Gated on CD34+ cells: to identify a population homogenous for CD34+ cells. d Ungated: to identify CD34+ cells which resemble the blast populations. e Ungated: to identify a CD38+ population from the total population. f Ungated: to identify a CD123+ population from the total population. g Dot plot: gated on CD34+ homogenous cells. h Density plot: gated on CD34+ homogenous cells. i Density plot: gated on CD34+CD38- cells to identify a population homogenous for the scatter. j Dot plot: gated on CD34+CD38- homogenous cells. k Density plot: gated on CD34+CD38- homogenous cells. l Density plot: gated on CD34+CD38- homogenous cells to identify a population homogenous for the scatter.

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