Effects of the p110α and p110δ dual inhibitor GDC-0941 in MCL cells. a Jeko-1, JVM-2, and Granta 519 cells were treated with GDC-0941 (1 μM) for 24 h, subjected to lysis, and analyzed by Western blot. Changes in the expression levels of p-AktSer473 and p-GSK3βSer9 are shown. Intensity of the immunoblot signals after background subtraction was quantified using ImageJ software, and the relative intensity compared with that of α-tubulin was calculated. Representative results from 3 independent experiments are shown. b-d Jeko-1, JVM2, and Granta 519 cells were treated with the indicated doses of GDC-0941 for 48 h. After treatment, inhibition of cell proliferation was determined by MTS assay (b), cytotoxic effects were detected trypan blue staining (c), and induction of cell cycle arrest was measured by flow-cytometric analysis by PI staining (d). Graphs represent average percentages ±SEMs from 3 independent experiments. * p < 0.05, ** p < 0.01.
