Western blot analysis of PI3K/Akt/mTOR signaling and cell cycle-related proteins. Jeko-1 and Granta 519 cells were treated with the indicated dose of PI3Kα inhibitor IV or IC87114 for 24 h, subjected to lysis, and analyzed by Western blot. Changes in the expression levels of p-Akt^Ser473, p-S6K^Ser240/244, cyclin D1, p-Rb^Ser780, p-GSK3β^Ser9 and p-4E-BP1^Thr37/46 are shown. Intensity of the immunoblot signals after background subtraction was quantified using ImageJ software, and the relative intensity compared with that of α-tubulin was calculated. Representative results from 3 independent experiments are shown.