Effects of PI3K isoform-specific inhibitors on cell viability in MCL cells. Jeko-1, JVM-2, and Granta 519 cells were treated with the indicated doses of PI3Kα inhibitor IV, IC87114, or LY294002 for 48 h. a Inhibition of cell proliferation was determined by MTS assay. b Cytotoxic effects were detected by trypan blue staining. Graphs at the top represent FACS data, while the bar graphs represent average percentages ±SEMs from 3 independent experiments. * p < 0.05, ** p < 0.01. c Apoptosis induction was measured by flow-cytometric analysis with annexin V/PI staining. Graphs at the top represent FACS data, while the bar graphs represent average percentages ±SEMs from 3 independent experiments. * p < 0.05, ** p < 0.01.