Examples of FC gating strategies applied to FNA in different lymphomas. a FSC versus SSC analysis gate. Gating on lymphocytes was sufficient to identify the neoplastic CD5⁺CD20⁺ B-cell population with monotypical bright expression of the lambda light chain in a mantle cell lymphoma case detected in a salivary gland. b Fluorescence versus FSC analysis gate. Gating on the slightly larger CD19⁺ B cells (in blue) enabled the isolation of neoplastic B cells displaying a lambda light chain in a case of MALT lymphoma of the thyroid (polyclonal B cells are in red). c Fluorescence versus fluorescence analysis gate. Gating on CD10⁺CD19⁺ B cells (in blue) was required to demonstrate the presence of a monotypical expression of kappa light chain of the neoplastic B cell population hidden among polyclonal B cells (in red) in a case of FL detected in the parotid gland.