Fig. 3
Example of a multiplane scan whole-slide image in which each of the planes has been condensed (or integrated) into a final single plane for viewing. This process identifies the best focused plane in each area and incorporates it into the final image. The upper figure is a condensed final image derived from a 9-plane scan whereas the bottom image is a single-plane scan. Note at the arrows significant improvement in focus of the cells in the 9-plane scan. Integration is not a final answer because it will not resolve groups of cells or cells overlapping one another, but it improves the focus over single-plane scanning as was illustrated in figure 1.

Example of a multiplane scan whole-slide image in which each of the planes has been condensed (or integrated) into a final single plane for viewing. This process identifies the best focused plane in each area and incorporates it into the final image. The upper figure is a condensed final image derived from a 9-plane scan whereas the bottom image is a single-plane scan. Note at the arrows significant improvement in focus of the cells in the 9-plane scan. Integration is not a final answer because it will not resolve groups of cells or cells overlapping one another, but it improves the focus over single-plane scanning as was illustrated in figure 1.

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