a Scheme of RUNX1 gene with the identified mutations marked. RHD, Runt homology domain, TAD, transactivation domain. b Expression ratio of RUNX1 mutant transcript (as a target gene) versus ABL1 reference gene at diagnosis served as 100% mutant value. Results of the expression ratio in follow-up time points presented as a percentage from diagnosis and calculated by relative ∆∆Ct method with correction for PCR efficiency. Dx, diagnosis, CR, complete remission.