a The G-banded karyotype showed the translocation t(5;8)(q32;p22). b FISH revealed a separated signal using a PDGRFB dual-color probe. c Agarose gel electrophoresis of the RT-PCR product of the PCM1-PDGFRB fusion transcript, using primers PCM1-F (5′-ATCGTAGTTCACAACAACCT-3′) in PCM129 exon and PDGFRB-R(5′-AGACTCAATCACCTTCCATC-3′) in PDGFRB12 exon. The predicted product size was 374 bp. The reciprocal fusion transcript PDGFRB-PCM1 was negative. d Bidirectional Sanger sequencing of the RT-PCR product confirmed that PCM1 exon 30 fused to PDGFRB exon 11.