... caused by cold temperature (CT) has been identified as an important mechanism contributing to enhanced platelet reactivity, detectable in freshly prepared platelets within 1 h of cold storage. The aim of this study was to confirm the effects of short-term refrigeration in platelets from apheresis-derived...

... metabolome to drive the development of novel storage additives. Key Message: In the near future, high-throughput testing of donor genomes via precision transfusion medicine arrays and metabolomics of all donated products will be able to inform the development and implementation of machine learning strategies...

...Kathryn A. Melzak; John L. Spouge; Clemens Boecker; Frank Kirschhöfer; Gerald Brenner-Weiss; Karen Bieback Background: Red blood cells (RBCs) stored for transfusions can lyse over the course of the storage period. The lysis is traditionally assumed to occur via the formation of spiculated...

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... of leukoreduction and gamma irradiation on the release of cf-DNA during storage. Methods: This is a prospective cohort study on 99 stored red cell components, randomly divided into three groups – buffy coat (BC)-depleted, leuko-filtered (LP), and irradiated (IR) packed red blood cells. Red cell supernatants were...

... of HSCs from 2007 to the present. The preclinical development of new cryoprotectants and new technology to eliminate cryoprotectants after thawing are discussed in detail. Additional cryopreservation considerations are included, such as cooling rate, storage temperature, and cell concentration...

...Kathryn A. Melzak; Stefanie Uhlig; Frank Kirschhöfer; Gerald Brenner-Weiss; Karen Bieback Summary Background: During storage of red blood cell (RBC) concentrates, the plasticizer di-2-ethylhexylphthalate (DEHP) that keeps the blood bags soft leaches out and can be taken up by the RBCs. DEHP...

...Astrid J.F. Thielen; Elisabeth M. Meulenbroek; Inge Baas; Robin Bruggen; Sacha S. Zeerleder; Diana Wouters Summary Background: In the Netherlands, red blood cells (RBCs) are allowed to be stored up to 35 days at 2-6 °C in saline-adenine-glucose-mannitol (SAGM). During storage, RBCs undergo several...

...-phosphate-dextrose (CPD) preserved whole blood, followed by up to 20 days refrigerated storage, and ii) whether Trolox supplemented to the blood bank-manufactured saline-adenine-glucose-mannitol (SAGM) preserved RBC units would offer better storage conditions compared with SAGM alone. Methods...

...) in polystyrene tubes and subjected to pre-storage irradiation (50 Gy) and to compare the properties of SAGM-preserved RBCs stored under experimental conditions (polystyrene tubes) with RBCs from standard blood bag storage. Methods: The percentage of hemolysis as well as the extracellular activity of LDH...

... into practice. Data management system Donors Haematopoietic cell transplantation Haematopoietic stem cells Quality management Stem cell collection Storage Transplantation 14 09 2011 28 10 2011 17 11 2011 © 2011 S. Karger AG, Basel 2011 Copyright / Drug Dosage / Disclaimer...

...Kelvin G.M. Brockbank; Eliza Rahn; Gregory J. Wright; Zhenzhen Chen; Hai Yao Background: The purpose of this research was to assess the extracellular matrix and chondrocytes of articular cartilage during refrigerated storage and to determine whether changes could be detected in the time frame...

...Jelena L. Holovati; Jason P. Acker Biopreservation is the process of maintaining the integrity and functionality of cells held outside the native environment for extended storage times. The development of red blood cell (RBC) biopreservation techniques that maintain in vitro RBC viability...

...Christoph Stoll; Willem F. Wolkers Storage methods, which can be taken into consideration for red blood cells and platelets, include liquid storage, cryopreservation and freeze-drying. Red blood cells can be hypothermically stored at refrigerated temperatures, whereas platelets are chilling...

... of Cryobiochemistry, Institute for Problems of Cryobiology and Cryomedicine, Ukraine Academy of Sciences, Kharkov, Ukraine c Cell, Tissue and Organ Preservation Unit, Department of Surgery & Liver Transplant Unit, UCL Medical School, Royal Free Hospital Campus, London, UK Keywords Biopreservation · Storage · Organ...

... of the collagenous stromal layer. Since human corneal endothelial cells do not readily proliferate, preservation of the endothelium is a primary aim of methods of corneal storage. Although some cryopreserved corneas have been transplanted successfully, the complexity of the cryopreservation technique and its...

... of the confounding ethical issues surrounding embryonic stem cells. An essential pre-requisite to the commercial and clinical application of stem cells are suitable cryopreservation protocols for long-term storage. Whilst effective methods for cryopreservation and storage have been developed for haematopoietic...

... before storage. Both aliquots were stored for 5 days, and GP lb, GMP140 (by flow cytometry) as well as LDH, pH, β-thromboglobulin, and PF4 were determined at days 1,3 and 5. During storage a loss of GP lb of about 25%, an increase of GMP 140 (50%) and LDH (30%) was observed. No difference between...