... encoded by GYP*Mur homozygote (do not express GPB, JENU-negative) receiving either Mi a -negative or Mi a -positive phenotype can produce anti-JENU, leading to difficulty in locating compatible donors. Genotyping for GYP*(B-A-B) hybrid alleles to predict the JENU-negative phenotype is alternatively...

..., instructions or products referred to in the content or advertisements. 2024 McLeod syndrome XK gene Null allele Genotyping McLeod syndrome (MLS) is a rare multisystem, X-linked recessive disorder caused by variations in the XK gene on chromosome X [ 1‒3 ]. The XK gene, comprising three...

... responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. 2024 ABO blood group Antigens Gene expression Genotyping Immunohematology Transcription factors MicroRNA The genetic code of the DNA...

... DEL D variant Alloimmunization Genotyping The DEL phenotype is defined as an extremely weak expression of the D antigen on red blood cells. Serologically, DEL is detectable only by adsorption and elution assay [ 1, 2 ]. There is no agglutination in the conventional indirect antiglobulin...

... Non-invasive prediction Cell-free DNA Genotyping Next-generation sequencing Alloimmunization against the K blood group antigen (ISBT KEL1 006001) is an important cause of hemolytic disease of the fetus and newborn with life-threatening fetal anemia [1, 2]. There is presently no possibility...

...Snezana Jovanovic Srzentic; Marko Lilic; Natasa Vavic; Ivana Radovic; Iva Djilas Introduction: The aim of this study was to investigate the allele and genotype frequencies of 8 human platelet antigen (HPA) systems among blood donors from the Blood Transfusion Institute of Serbia and to compare them...

...>G substitution, 0.4% of the Syrian probands were heterozygous for DO * 01/DO * 01.-05 , a genotype that was lacking in German donors. Whereas the KEL * 02.06 allele coding for the Js(a) phenotype was missing in Germans; 0.8% of the Syrian donors carried 1 copy of this allele. 1.8% of the Syrian...

... Genotyping ABO blood group-caused hemolytic disease of the fetus and newborn (HDFN) is very frequent but only rarely severe [ 1-9 ]. The ABO blood group system is the only system in which natural IgM antibodies against the corresponding antigens are universally present from a young age...

...Steffen Klasberg; Vineeth Surendranath; Vinzenz Lange; Gerhard Schöfl The advent of next generation sequencing (NGS) has altered the face of genotyping the human leukocyte antigen (HLA) system in clinical, stem cell donor registry, and research contexts. NGS has led to a dramatically increased...

... samples were typed for the FORS1 Ag using a monoclonal anti-Forssman antibody. The GBGT1 gene was genotyped by DNA sequencing (all adult samples) or by using amplification refractory mutation system PCR (newborn samples). Results: All of the studied samples were negative for the FORS1 Ag by serologic...

... thrombocytopenia (NAIT). The antigens are defined by single nucleotide polymorphisms (SNPs) in the glycoprotein genes, and the antigen status can be determined by genotyping the SNPs. However, genotyping is time-consuming and costly depending on the method and sample throughput. Here, we tested the reliability...

..., and 11 HNA. Furthermore, we describe a feasible method for fast molecular screening of the HNA-2 null phenotype. All data were statistically evaluated regarding genotype distribution. Allele frequencies were compared to ExAC data from non-Finnish Europeans. Results: Up to now more than 2,000 non-selected...

... FCGR3B and FCGR3A complicates the development of genotyping assays. With about 1-2 × 10 5 copies per cell the low-affinity IgG1/IgG3 FcγRIIIb mediates clearance of immune complexes and phagocytosis of opsonized microorganisms [ 17,18,19 ]. The membrane proximal domain is critical for IgG Fc binding...

..., the allele a frequencies for HPA-1, -2, -3, -5 and -15 were 0.8575, 0.8400, 0.6225, 0.8525 and 0.5825 respectively. The genotypes with higher frequencies were a/a (72-74%), except for HPA-3 and -15, whose heterozygous a/b genotypes were shown to be more prevalent (43.5 and 44.5%, respectively). Conclusion...

...Yann Fichou; Cédric Le Maréchal; Virginie Scotet; Déborah Jamet; Claude Férec Summary Background: Although systematic blood group genotyping of patients/donors is virtually possible, serological studies remain the gold standard to identify samples of clinical interest that may be further genotyped...

...Francesca Drago; Katerina Karpasitou; Laura Spinardi; Loretta Crespiatico; Mario Scalamogna; Francesca Poli Background: In a previous publication we described a method for Jk a /Jk b , Fy a /Fy b , S/s, K/k, Kp a /Kp b , Js a /Js b , Co a / Co b , and Lu a /Lu b genotyping based on a microsphere...

... research, with a wide spectrum of methods being available. One of the most versatile approaches relies on primer extension to genotype single alleles, microsatellite repeat lengths or the methylation status of a given cytosine. Optimized methods comprising intelligent primer design and proper nucleotide...

... Colton blood group system Kell blood group system Kpb Lutheran blood group system Genotyping Rare blood donor High-throughput donor testing Review Article · Übersichtsarbeit Transfus Med Hemother 2009;36:199 203 DOI: 10.1159/000218110 Received: February 11, 2009 Accepted: May 5, 2009 Published...

...Christof Jungbauer The provision of suitable blood units for patients carrying clinically significant antibodies to high-frequency antigens (HFAs) is a special challenge for blood establishments. Typing of donors and screening for HFA-negative individuals is increasingly performed by genotyping...

... was compiled for a performance comparison of the three RhD and ABO serological typing methods MDmulticard (Medion Diagnostics), ID-System (DiaMed) and ScanGel (Bio-Rad). The final test panel included standard and variant RHD and ABO genotypes, e.g. RhD categories, partial and weak RhDs, RhD DELs, and ABO...