Background: The aim of the present study was to detect the occurrence of viable Toxoplasms or the presence of their DNA. Red blood cell concentrates of specific IgM-positive donors were tested in order to estimate if such blood products may have an infectious potential when transfused. Material and Methods: Aliquots of 1,000 red cell concentrates were used to inoculate in vitro cell cultures which were examined microscopically 6-7 days later with fluorescent staining for Toxoplasma gondii. Additionally, after a DNA isolation procedure, a polymerase chain reaction (PCR) was performed using a part of the sequence of the toxoplasma B1 gene as a target. Dilutions of T. gondii tachyzoites with the red cell concentrate were used as positive controls. Results: Tachyzoites could be detected by in vitro culture even when the inoculum statistically contained only one parasite. About 3 tachyzoites yielded a positive result with the PCR method. All red cell samples investigated gave negative results both in cell culture and PCR. Conclusion: The risk of transmission of Toxoplasms by transfusing red cell concentrates from specific IgM-positive donors can be considered unlikely.

This content is only available via PDF.
Copyright / Drug Dosage / Disclaimer
Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.
You do not currently have access to this content.