...Carl Q.-Y. Zeng; Elliot Alpert Various coefficients in radioimmunoassays are expressed in a series of equations. These equations provide an approach to estimating the number of epitopes on a multivalent antigen as well as a macromolecular topogram. In this paper, pig insulin (Ins), horse myoglobin...

... epitope diversity. In this study we describe the heterologous expression of a CA 125 repeat unit (R11) and an analysis of its epitope topography. Methods: R11 was expressed using a baculovirus approach and purified from culture supernatants by sequential ion exchange chromatography. Monoclonal antibody...

...Y. Kang; E. Matsuura; T. Sakamoto; M. Sakai; S. Nishi Thirty-six monoclonal antibodies (MAbs) against human alpha-fetoprotein (AFP) were analyzed for the location of their epitopes by reacting them with a set of yeast recombinant AFP proteins using ELISA. Recombinant AFP proteins containing either...

... antibodies (MAb) are used for the diagnosis and therapy of CEA-positive tumors. The five major nonoverlapping epitopes (Gold 1–5) have been broadly correlated with the domain organization, but there is no precise localization of the epitopes at the sequence level. In an attempt to identify the peptide...

...-1), the epitope specificities of 30 different monoclonal antibodies against cytokeratins 8, 18 and 19 were determined. This second report presents the immunohistochemical profiles of these antibodies using human appendix and normal skin for evaluation. Each antibody was tested by one or two...

... or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. Alpha fetoprotein Antibody affinity Antibody specificity Epitope Monoclonal antibody ISOBM TD-2 Workshop Report Tumor Biol 1998;19:293 300 K. Nustad E. Paus B. Kierulf O.P. Børmer...

...Jian Dai; W. Jeffrey Allard; Gary Davis; Kwok K. Yeung We evaluated 56 monoclonal antibodies (MAbs), submitted to the ISOBM TD-4 Workshop, for changes in binding following desialylation of the MUC1 molecule and for epitope specificity. Antibody binding of MAbs was assayed by an ELISA method using...

...Yi Cao; Uwe Karsten; Jo Hilgers The epithelial mucin MUC1 is heavily but differently glycosylated depending on the origin and developmental status of the tissue, which greatly influences the reactivity of monoclonal antibodies (MAbs). A partial characterization of their epitopes is possible by mild...