... effect, demonstrating a biphasic AFP activity. Cell pretreatment with the transcriptional inhibitor actinomycin D had no measurable influence on AFP cytotoxicity. These findings demonstrate that protein synthesis is not required for this mechanism of cell death. The charcoal-treated ligand-free eAFP...

...Lydia N. Semenkova; Elena I. Dudich; Igor V. Dudich; Ludmila N. Shingarova; Vyacheslav G. Korobko Human hepatocarcinoma HepG2 cells are known to be insensitive to tumor necrosis factor (TNF) cytotoxicity. In this report, preliminary washing of HepG2 cells with serum-free medium to remove endogenous...

...G. Basha; P. Yap; F. Penninckx The trypan blue exclusion test, the MTT test and an immuno-staining test for apoptosis were performed before and after incubation of SW620 human colonic carcinoma cells with different cytotoxic agents (CTAs) in order to assess tumor cell viability and CTA efficacy...

...G. Basha; F. Penninckx; K. Geboes; P. Yap Previously, we have assessed the efficacy of different cytotoxic agents on the viability of SW620 human colonic carcinoma cells in vitro. In this study, we have investigated the tumoricidal efficacy of some antiseptic agents on SW620 human colonic carcinoma...

...M. de la Fuente; M.C. Alonso; R. Solana; J. Peña Antibody-dependent cell-mediated cytotoxicity (ADCC) mediated by peritoneal macrophages and splenic lymphocytes was studied in young (15 weeks) and old (45 ± 5 weeks) preleukemic AKR/J mice and leukemic (55 ± 5 weeks) AKR/J mice. This strain...

... killer (NK) cell-mediated cytotoxicity against cancer cells. Methods: Head-and-neck squamous cell carcinoma (HNSCC) cell lines were analyzed for the expression of CD47 and susceptibility to NK cell-mediated killing. Cytolytic activity was assessed by 51 Cr-specific release assays and by measuring...