The carbohydrate specificity of 57 MAbs submitted to the ISOBM TD-4 Workshop on MUC1 were investigated by two versions of ELISA, direct binding and inhibition of binding. The following free saccharides and their polyacrylamide conjugates (Sug-PAA) were used: tetrasaccharides – SiaLex , SiaLea; trisaccharides – Lex, 3′HSO3Lex, Lea, 3′HSO3Lea, 3′SiaLac, Atri, Btri; a number of disaccharides including TF, Hdi, SiaTn, LactNAc, and monosaccharides. It was shown that MAbs 143 and 167 interacted only with SiaLex, MAbs 127 and 128 only with Lex. Antibodies 123 and 164 interacted preferably with Lea but also recognized Lec. Antibody 151 recognized αGalNAc (Tn) and cross-reacted with βGalNAc. Antibody 157 displayed high affinity to Atri and Atetr (type 1). Neither anti-TF nor anti-SiaTn antibodies were revealed.

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