The advent of high-throughput technologies to analyze RNA expression levels and transcript structure has brought renewed attention to the age-old question of what differentiates males from females. In Drosophila, the characterized somatic sex determination cascade includes proteins implicated in the regulation of pre-mRNA splicing as well as at least 2 transcription factors at its base. Both DNA microarrays and RNA-Seq have been applied in a number of studies to determine the identities, expression levels and structure of transcripts expressed differentially in the 2 sexes, with remarkably divergent results in the number, structure and identity of affected transcripts. We briefly summarize these reports and discuss the reasons for the apparent discrepancies based upon the different conditions used for sample preparation and data analysis.

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