... was verified by luciferase luminescence measurements. Results: H 2 O 2 inhibited HaCaT cell proliferation, migration, and promoted cell apoptosis, accompanied with the downregulation of miR-211. H 2 O 2 led to inflammatory response and oxidative damage to HaCaT. miR-211 promoted proliferation and migration...

..., while ceramides (Cer) produced from SM on plasma membranes serve as a lipid mediator that regulates cell proliferation, differentiation, and apoptosis. In the skin, SM also is a precursor of Cer, an important constituent of epidermal permeability barrier. We investigated the role of epidermal SM...

... in the epidermis by a tightly controlled equilibrium between cell proliferation and cell differentiation. Squamous differentiation is initiated by inhibiting the proliferation of epidermal keratinocytes followed by the induction of squamous-specific functions. Aberrant control of this process results in abnormal...

... involved in these two opposing processes remain unclear. Methods: We used normal human keratinocytes (NHK), the human keratinocyte HaCaT cell line and human epidermal carcinoma cells (A431 cell line) to investigate potential pathways involved in the effects on cell proliferation and growth inhibition...

... supplemented with 3% charcoal-stripped fetal calf serum and 0.3 m M Ca 2+ . Proliferation was measured by the dimethylthiazolyl-diphenyl-tetrazolium-bromide assay. Then differentiation was determined in the same culture, performing a cell ELISA for transglutaminase type 1. All-trans-RA, 9-cis-RA and CD2809 had...

...Charles S. Harmon; Thomas D. Nevins; Janet Ducote; Diane Lutz We have used a series of bisindolylmaleimide selective pro-tein-kinase C (PKC) inhibitors to investigate the role of this enzyme in the regulation of cell proliferation in mouse hair follicle organ cultures. Mouse whisker follicles were...

..., especially the fiavonoid fractions: quercetin, kaempfe-rol, sciadopitysin, ginkgetin, isoginkgetin, on the proliferation of normal human skin fibroblast in vitro measured by MTT (3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyl-tetrazolium bromide) assay and direct hemocytometer cell count. Furthermore, increased...

...Gerard J. Gendimenico; James A. Mezick Retinoids exert profound pleiotropic effects in skin, affecting many aspects of cell differentiation and proliferation. For this reason, retinoids have prominent pharmacological effects on major skin cells (keratinocytes, dermal fibroblasts, melanocytes...

...Janet A. Fairley; Peter D. Fung; Nancy M. Ewing Calmodulin, a major calcium-binding protein, is important in the regulation of cell proliferation. In human keratinocytes we found that culture confluence was accompanied by a decrease in calmodulin content. Ciclosporin A, 1–10 μg/ml, was found...

... response together with the changes of LTB 4 levels in lesional skin biopsy specimens, and urinary leukotriene E 4 (LTE 4 ) excretion were evaluated. In addition, markers of inflammation, proliferation and keratinization were studied immunohistochemically. No change in clinical scores or lesional LTB 4...

... of dithranol on epidermal proliferation, keratinization and inflammation was examined using immunohistochemistry. Punch biopsies from 6 volunteers who applied dithranol 0.5% in petrolatum were taken before application, after 48 and 96 h. Biopsies were processed for assessing epidermal proliferation by Ki67...

... responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. In vitro assay Keratinocytes Attachment Proliferation Growth stimulation Growth inhibition Toxicity Original Research Article Skin...

... epidermal diseases requiring no immunosuppression. 4 07 1989 6 10 1989 31 3 2009 Ciclosporin H Epidermal keratinocytes Proliferation DNA synthesis © 1989 S. Karger AG, Basel 1989 Copyright / Drug Dosage / Disclaimer Copyright: All rights reserved. No part...

... or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. Anagen hair Proliferation DNA flow cytometry Baldness, male pattern Hirsutism...

... skin regions. In addition, the effect of spironolactone on the proliferation of androgen-stimulated human sebocytes derived from facial skin was evaluated. Testosterone (1O -11 to 10 -5 M ), 5α-dihydrotestosterone (10 -11 to 10 -5 M ) and spironolactone (10 -12 to 10 -7 M ) were added for 10 days...

... and conventional fluorinated congeners, demonstrating similar anti-inflammatory activity in vivo, on biosynthetic capacities of human fibroblasts. Fibroblasts were kept in monolayer cultures and exposed to different concentrations of the active compounds for 6 days to analyze the influence on proliferation...

... to explain. Methods: We analyzed the effect of all- trans retinoic acid (all- trans RA), 13- cis retinoic acid (13- cis RA), ergocalciferol and cholecalciferol in keratinocyte cultures established from adult donors, on the cell proliferation by means of [ 3 H]thymidine incorporation and apoptosis after...

... that the biochemical basis for the mechanism of action of dithranol at the molecular level is related to the redox activity leading to the production of active oxygen species, which include singlet oxygen, superoxide anion radical and hydroxyl radical. Some authors suggest that epidermal proliferation...

... difference between the two studies is the late phase in the single-challenge group after 8 days, showing a longer-lasting response in the uninvolved skin compared to normal skin with respect to proliferation and inflammation markers. A controlled and synchronised irritation by dithranol might induce anti...

... counteraction to this well-known irritation. Therefore, we studied the effect of single and repeated applications of dithranol on normal human skin. Besides a clinical evaluation, we studied aspects of epidermal proliferation, differentiation and inflammation. On day 2, after single dithranol challenge, we...