...F. Raffali; A. Rougier; R. Roguet The metabolism of xenobiotics by the epidermis can be studied in vitro by using keratinocyte cultures. We present a simple and rapid method for determining the activity of cytochrome P450-dependent enzymes (ethoxycoumarin-O-de-ethylase, ECOD, and ethoxyresorufm-O...

..., but is a very potent inhibitor of endogenous vitamin A metabolism in human epidermal cells. To further extend this observation, we studied the effect of 12 different retinoids on the metabolism of [ 3 H]retinol ([ 3 H]ROH) in HeLa cells, previously shown to produce constant levels of 3,4-didehydroretinol (ddROH...

...Margaret E.K. Kraeling; Raymond J. Lipicky; Robert L. Bronaugh The effect of dose and enzymatic inhibition on the percutaneous absorption and metabolism of benzocaine was studied in vitro in the hairless guinea pig. At the dose level of 2 μg/cm 2 , benzocaine was rapidly absorbed and extensively...

...Naureen A. Shaikh; John I. Ademola; Howard I. Maibach Clinical and in vitro evidence suggests that the physicochemical properties of the skin influence the process by which drugs are transported through skin. The effects of skin storage, preparation and pretreatment on the permeation and metabolism...

..., quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. Cytochrome P-450 Reconstructed epidermis Metabolism Imidazole Original...

... acid analogues acitretin and arotinoid, and only slightly inhibited (minus 25%) by 4-(N-hydroxyphenyl)retinamide. Addition of citral and ketoconazole, substances known to interfere with the oxidative metabolism of vitamin A, diminished the formation of 3,4-didehydroretinol by 70 and 95%, respectively...

... a glycolic acid N-conjugate of ethyl 4-ami-nobenzoate, was found at about 100 times higher concentration after arterial than after dermal drug application, indicating that the quantitative metabolic patterns in skin differ depending on whether the xenobiotic reaches the enzymes of the skin by cutaneous...

...J. Barth; K.H. Lehr; H. Derendorf; H.W. Möllmann; T. Höhler; G. Hochhaus Prednicarbate (PC) is a nonhalogenated derivative of prednisolone which is used for the local treatment of corticoid-sensitive skin diseases. In this study, the pharmacokinetics and the metabolism of PC in humans...

... Skin concentrations Plasma concentrations Acne Metabolism Skin Pharmacol 1988;1:237-245 © 1988 S. Kargcr AG, Basel 1011 -0283/88/0014-0237S2.75/0 Concentrations of Isotretinoin and the Arotinoid Ro 15-0778 in Plasma and Tissues of Hamsters and Rats1 Floie M. Vane, Sarita S. Chari, James F. Hurley...

...Terence Kealey; Rebecca Williams; Michael P. Philpott On maintenance in supplemented Williams E medium, human hair follicles grow at the normal rate, and retain their normal anagen morphology, for up to 10 days. This permits us to study their metabolism under near-physiological conditions. The ATP...

...U. Münster; S. Hammer; U. Blume-Peytavi; M. Schäfer-Korting Since the limited knowledge of cutaneous drug metabolism can impair the development of specifically acting topical dermatics and transdermal application systems, the cell-type-specific androgen metabolism in human skin and its inhibition...

... times larger than that for ethanol dehydrogenation. The K I of 4-methylpyrazole for alcohol dehydrogenase from horse liver was 0.6 (3-phenoxybenzyl alcohol) vs. 0.04 µ M (ethanol). The K I for ethanol in baker’s yeast was 470 µ M . In conclusion dehydrogenation is an important metabolic pathway...

... responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. Retinoids Absorption Metabolism Rabbit Skin Original Research Article Skin Pharmacol Appl Skin Physiol 1998;11:241 249 Received: Febr. 27...