... such as 5-fluorouracil (5FU) induce intrinsic apoptosis. Objectives: To investigate the effects of TNFα on apoptosis in malignant and normal human keratinocytes. Methods: Human cutaneous squamous cell carcinoma (SCC) cell line SCC-13 and immortalized human keratinocytes HaCaT as well as primary normal human...

... at baseline in keratinocytes may predict responsiveness to TGC in patients with CVD. Methods: In this retrospective cohort study, clinical and biological data of 25 women with a histological diagnosis of chronic vulvar eczema were analyzed. Randomization was done according to the responsiveness to TGC...

... trials and non-interventional studies in patients with abrasions. 6 01 2020 30 05 2020 17 7 2020 [email protected] Comfrey Symphytum × uplandicum Boraginaceae Skin regeneration Keratinocytes © 2020 S. Karger AG, Basel 2020 Copyright / Drug Dosage...

... or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. Corneocytes Keratinocytes Cell maturation Cell nucleus Nucleoid Bacteria Skin barrier function Since the 19th century, it has been widely accepted that the mammalian skin...

... responses of PM10-exposed keratinocytes. Methods: Primary human epidermal keratinocytes were exposed in vitro to PM10 in the absence or presence of punicalagin and (–)-epigallocatechin-3-gallate (EGCG), which are the major polyphenolic antioxidants found in pomegranate and green tea, respectively. Assays...

... to in the content or advertisements. Vitamin D Skin Keratinocytes Psoriasis Atopic dermatitis The human skin acts as site of synthesis of vitamin D and also as target organ for the biologically active form of this vitamin. Vitamin D affects multiple functions in the skin ranging from keratinocyte...

...Iga Hołyńska-Iwan; Inga Dziembowska; Paulina Smyk; Magdalena Lampka; Dorota Olszewska-Słonina Acute, adverse skin effects to capsaicin can be activated by inhibition of sodium transport not only in nociceptive neurons, but also in keratinocytes. The aim of the current study was to describe...

... therapies, but medicinal plants are increasingly employed. Objective: To investigate the protective role of white willow bark (WWB) and 1,2-decanediol (DD) on the damage caused by lipopolysaccharides (LPS) on human adult keratinocytes (HaCaT). Methods: HaCaT were exposed to LPS alone or in association...

.... Here, we investigated the effects of oral CP administration on gene expression in hairless mouse skin and of prolyl-hydroxyproline (Pro-Hyp), a collagen-derived dipeptide, on gene expression in a coculture of mouse skin keratinocytes and fibroblasts. Using microarray analysis, we found that oral...

... have not been elucidated. Objective: We investigated whether WG promotes the migration and/or proliferation of cultured human keratinocytes (CHK) or dermal fibroblasts in parallel with increases in lipid synthesis during in vitro wound healing. Methods: CHK or fibroblasts were treated with 1–1,000 µg...

...S. Sørensen; H. Sølvsten; Y. Politi; K. Kragballe Keratinocytes express receptors for 1,25-dihydroxy vitamin D 3 [1,25(OH) 2 D 3 ], all-trans-retinoic acid (all-trans-RA), 9-cis-retinoic acid (9-cis-RA) and triiodothyronine (T 3 ). The vitamin-D receptor (VDR) can act as a transcription factor...

...Donald A. Vessey; Kyung-Hee Lee; Eva Lau Sixteen bile acids were tested at a concentration of 50 µ M for their effect on growth of preconfluent cultures of proliferating keratinocytes. Monohydroxy bile acids (3-Β-hydroxy-Δ5-cho-lenate and lithocholate) stopped the accumulation of protein...

...F. Raffali; A. Rougier; R. Roguet The metabolism of xenobiotics by the epidermis can be studied in vitro by using keratinocyte cultures. We present a simple and rapid method for determining the activity of cytochrome P450-dependent enzymes (ethoxycoumarin-O-de-ethylase, ECOD, and ethoxyresorufm-O...

...Kohji Maruyama; Jian-Zhong Zhang; Yoshimichi Nihei; Ichiro Ono; Fumio Kaneko It is known that keratinocytes produce and secrete interleukin-1 (IL-1) de novo and that this process can be enhanced by various stimulators. IL-1 has been shown to be a potent proin-flammatory cytokine which mediates...

... kinase C (PKC) is considered to mediate cellular responses to other epidermal tumor promotors, we wished to investigate the relationship between BzPO and PKC in cultured human epidermal keratinocytes (NHEK). We assayed (a) direct effects of BzPO on PKC activity in a cell-free system using semipurified...

...Yimin Xiong; Charles S. Harmon We have used fura-2/AM to investigate the effect of diazoxide on the cytosolic calcium concentration (Ca i ) in primary mouse keratinocyte cultures. Treatment of keratinocytes with 100 µ M diazoxide induced a transient peak in Ca i , followed by a sustained elevation...

...Gerard J. Gendimenico; James A. Mezick Retinoids exert profound pleiotropic effects in skin, affecting many aspects of cell differentiation and proliferation. For this reason, retinoids have prominent pharmacological effects on major skin cells (keratinocytes, dermal fibroblasts, melanocytes...

... and systemic treatments. In this preliminary study we investigate the effect of CyA on the tPA mRNA transcription on A431 keratinocytes cell line. Sub-confluent A431 cell cultures have been treated with CyA at in vivo relevant concentrations (10, 7.5, 5 µg/ml) for 48 h. Northern blot analysis of total RNA...

... on the proliferation of keratinocytes is discussed controversially. The second messenger cyclic adenosine monophosphate (cAMP) has been regarded as a regulator for cell growth and proliferation for 20 years. Hyperproliferation of many cells and particularly of psoriatic keratinocytes was speculated to be due...

... by keratinocytes making possible the study of dermoepidermal interactions. In this in vitro model, close to the in vivo situation, the skin cells interact with matrix molecules and are submitted to cell to cell communications. Under the influence of these interactions they differentiate. The predictivity...