... psoriasis-like mouse model and IMQ-induced differentiated human keratinocytes (HaCaT) cells. Methods: BALB/c mice were treated with IMQ to construct a psoriasis-like mice model. PASI score and HE staining were used to observe pathology injury of skin tissue. The secretion of inflammatory factors...

...Yitao Wang; Feifei Jiang; Fang Chen; Dapeng Zhang; Jian Wang Introduction: Psoriasis is an immune-mediated polygenic inflammatory skin disease in which keratinocyte proliferation is an important mechanism. The study investigated the role and regulatory relationship between lncRNA XIST and miR-338...

.... Differentiation Keratinocyte Proliferation Skin barrier function Sphingomyelin Sphingomyelin synthase National Institutes of Health (NIH) 10.13039/100000002 Sphingomyelin (SM), which is a phosphosphingolipid, is a ubiquitous constituent of cellular membranes. During activation...

..., on human cells using a 3D skin model. Methods: CAP treatment of 3D skin models consisting of a keratinocyte-containing epidermal layer and a fibroblast/collagen dermal matrix was performed using the Tigres plasma MEF technology. To evaluate the effects on the 3D skin models, the following plasma parameters...

... them from oxidative damage. Coriandrum sativum L. (coriander; CS) is a commonly consumed food and a traditional phytomedicine in Asia and Europe. In this study, we examined the protective effects of a standardized CS leaf extract against oxidative stress in human HaCaT keratinocytes. Methods...

... involved in these two opposing processes remain unclear. Methods: We used normal human keratinocytes (NHK), the human keratinocyte HaCaT cell line and human epidermal carcinoma cells (A431 cell line) to investigate potential pathways involved in the effects on cell proliferation and growth inhibition...

... in the epidermis. The present studies were undertaken to compare the uptake and incorporation into cellular lipid of these fatty acids and to determine whether they compete with one another in these processes in relation to keratinocyte differentiation. The incorporation of [ I4 C]LA and/or [ 14 C]AA...

...Donald K. MacCallum; John H. Lillie Oral and epidermal rat keratinocytes when cultured on a matrix of type I collagen fibrils at the interface between the gaseous and liquid phases of a culture form a highly ordered stratified squamous epithelium. Autoradiographic studies of cells labeled...

...Leslie C. James; Alison M. Moore; Larry A. Wheeler; Gilliam M. Murphy; Pauline M. Dowd; Malcolm W. Greaves Transforming growth factor Α (TGFΑ) is a keratinocyte-growth-stimulating factor which may have a role in epidermal hyperproliferation, psoriasis, and wound healing. Since increased epidermal...

... Acid Receptor Antagonist in the Human Epidermal Cell Line SCL-II Key Words Kératinocyte 12-HETE binding DuP 654 Abstract The lipoxygenase inhibitor 2-phenylmethyl-l-naphthol (DuP 654) has been shown to be an active anti-inflammatory drug in a murine skin inflammation model. Since 12-HETE is as­ sumed...

... are described: keratinocyte and fibroblast cultures grown under submerged culture conditions, epidermal equivalents, skin equivalents and freshly isolated skin. Biomarkers such as IL-1α, IL-6, IL-8, PGE2, SKALP, HSP70 and kinases are described along with changes in metabolic activity (MTT assay) and cytosolic...

...A. Santiago-Walker; L. Li; N.K. Haass; M. Herlyn Melanocytes in human skin are intricately regulated by keratinocytes and the surrounding stroma. The development of melanoma is thought to arise from disrupted melanocyte homeostasis. It is now known that microenvironment plays a major role...

... epidermal keratinocytes. The production of IL-1α, CXCL10, and CCL17 and the expression of CD54 were significantly suppressed by the addition of bepotastine. Bepotastine exerts its antiallergic action on keratinocytes by suppressing the production of certain proinflammatory cytokines, both Th1 and Th2...

..., glutathione S-transferase (GST). The enzyme activities were determined in cultured keratinocytes, reconstructed epidermal models and samples of human epidermis or hair follicle. EROD activity was detected in cultured keratinocytes and was induced by 3-methylcholanthrene (3-MC) and β-naphthoflavone. The level...

.... We also determined the inter- and intrabatch variability and demonstrate that NQR activity can be significantly inhibited by dicumarol treatment. The NQR activity in reconstructed epidermis is similar to that in human epidermis and lower than in cultured keratinocytes. Therefore reconstructed...

...R. Weller; T. Billiar; Y. Vodovotz Ultraviolet radiation (UV) induces apoptosis in keratinocytes by both p53- and death receptor-dependent pathways. It also generates free radicals in keratinocytes, including the synthesis of nitric oxide (NO) by constitutive and inducible NO synthases (NOS...

.... Transglutaminase Cornified envelope Keratinocyte Differentiation Cross-linking Isopeptide bond Apoptosis 12 2 2000 Original Research Article Skin Pharmacol Appl Skin Physiol 2000;13:17 30 Received: June 8, 1999 Accepted: June 9, 1999 Transglutaminase Reactivity of Human Involucrin Adam Lamberta...

...Jürgen Göhring; Dirk Fröde; Wolfgang Wohlrab The effects of decylhydroperoxide (DHP) on proliferation were assessed in HaCaT keratinocytes. DHP, a model compound of lipid peroxidation, reduced proliferation dose dependently. The analyses of cell number, DNA synthesis and cell cycle distribution...

...(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. Transglutaminase Cornified envelope Keratinocyte Epidermis Original Research Article Skin Pharmacol Appl Skin Physiol...

...Thomas Mammone; Ken Marenus; Daniel Maes; Richard A. Lockshin The terminal differentiation of human epidermal keratinocytes is a complex morphological and biochemical shift from a mitotically active cell to an inert protein cross-linked envelope. This transition is a clearly predetermined cell...