.... Objective: This study aimed to determine lncRNA expression changes in human dermal fibroblasts (HDF) induced by repeated UVA irradiation and to explore correlations between lncRNA and skin photoaging prognosis. Methods: In the UVA-HDF group, HDF were subjected to repeated UVA irradiation (10 J/cm 2 UVA...

..., we studied their effect on dermal fibroblasts and extracellular matrix (ECM) production. Methods: S100 peptides, ECM regulation and distribution were analysed in normal and keloid tissue by quantitative PCR (qPCR), immunoblotting and immunofluorescent staining. Isolated dermal fibroblasts were...

... using objective measurements. In addition, the underlying molecular mechanisms were investigated using cultured keloid-derived fibroblasts. Materials and Methods: Four patients received BTA (doses varying from 70 to 140 Speywood units per session) injected directly into their keloids every 2 months...

... have not been elucidated. Objective: We investigated whether WG promotes the migration and/or proliferation of cultured human keratinocytes (CHK) or dermal fibroblasts in parallel with increases in lipid synthesis during in vitro wound healing. Methods: CHK or fibroblasts were treated with 1–1,000 µg...

... as a result of chronic UV irradiation were detected by immunohistochemistry methods. Protein cathepsin expressions in UVA-induced premature senescence in fibroblasts in vitro were detected by Western blot technique. Cathepsin mRNA expression in photoaged skin and fibroblasts was detected by real-time reverse...

...Gerard J. Gendimenico; James A. Mezick Retinoids exert profound pleiotropic effects in skin, affecting many aspects of cell differentiation and proliferation. For this reason, retinoids have prominent pharmacological effects on major skin cells (keratinocytes, dermal fibroblasts, melanocytes...

...Marc Heckmann; Thomas Krieg During recent years, considerable progress in the field of connective-tissue research has brought about detailed information on the behaviour of fibroblastic cells under culture conditions and in in vivo situations. Well-characterized biological functions of fibroblasts...

... and conventional fluorinated congeners, demonstrating similar anti-inflammatory activity in vivo, on biosynthetic capacities of human fibroblasts. Fibroblasts were kept in monolayer cultures and exposed to different concentrations of the active compounds for 6 days to analyze the influence on proliferation...

...L. Dubertret By mixing normal human fibroblasts with collagen type I + III and taking advantage of the capacity of fibroblasts to contract collagen fibers, it is possible to reconstruct in vitro a simplified equivalent of the human dermis. This tridimensional culture system can be covered...

... by foreskin fibroblasts and (2) an epidermis regenerated from normal human keratinocytes seeded onto the dermal equivalent. The dermal substrate contains type I and III collagen and glycosaminoglycans (GAGs) cross-linked by chitosan. Fibroblasts seeded into the porous structure of the dermal substrate provide...

...-retinoic acid stimulates the proliferation of growth-arrested human keratinocytes and fíbroblasts in culture. In the present studies, all-trans-retinoic acid was compared to three other retinoids – 13-cis-retinoic acid, P-[(E)-2-(5,6,7,8-tetrahydro-5,5,8,8-tetramethyl-2-napthalenyl-l-propenyl] benzoic acid...

...L. Cézanne; F. Gaboriau; M. Charveron; P. Morlière; J.F. Tocanne; L. Dubertret In this preliminary report, dealing with the biological properties of AvÈne spring water, we investigated its effects on membrane properties of cultured human skin fibroblasts used as a model cell system. It is shown...

...A. Er-raki; M. Charveron; J.L. Bonafé We studied the superoxide anion activity of psoriatic and normal dermal fibroblasts in culture. Superoxide anion production was measured by the technique of Pick and Mizel in fibroblasts cultured from biopsies of involved and uninvolved skin of patients...

... human skin fibroblasts. The experiments have been performed with either pure selenium or a spring water containing selenium and other trace elements (zinc and strontium). For cells cultured in a standard medium containing 10% fetal calf serum, no effect of selenium or spring water addition...

... techniques used in various disciplines have been gaining importance. A wide variety of cell lines are commonly used: cultured fibroblasts from human skin, buccal mucosa, periodontal membrane or embryonic lung; epithelial and HeLa cells; cultures of human keratinocytes and HaCaT cells; different murine cell...

... metalloproteinase-1 (MMP-1; interstitial collagenase). When human epidermal keratinocytes were treated with the EGF-RTK antagonist in monolayer culture, growth inhibition was seen (ED 50 = approximately 0.06 µ M ). When dermal fibroblasts were exposed to the EGF-RTK antagonist in monolayer culture, proliferation...

.... Prednicarbate Glucocorticoids Transactivation Keratinocytes Fibroblasts 29 04 2002 27 09 2002 11 4 2003 Skin Pharmacology andApplied Skin Physiology Original Research Article Skin Pharmacol Appl Skin Physiol 2003;16:143 150 DOI: 10.1159/000069754 Received: April 29, 2002 Accepted...

..., desoximetasone (DM) and betamethasone 17-valerate (BMV). Isolated foreskin keratinocytes served for in vitro investigations of anti-inflammatory processes in the epidermis, fibroblasts of the same origin were used to investigate the atrophogenic potential. Inflammation was induced by TNFα, resulting...

... of TCA and GA on human keratinocytes and the influence of the released epithelial mediators on collagen and matrix metalloproteinases (MMPs) production by human dermal fibroblasts. Method: Cultured keratinocytes were treated by TCA and GA at 10 mg/ml brought to pH 3, 5 and 7, and the conditioned media...

...Shingo Sakai; Tetsuya Sayo; Shuji Kodama; Shintaro Inoue We examined the effects of N -methyl-L-serine (NMS), an amino acid derivative, on hyaluronan (HA) synthesis in human skin fibroblasts. NMS (1–10 m M ), but not L-serine, stimulated the incorporation of [ 3 H]glucosamine into HA dose...