Abstract
Introduction: We aimed to investigate the effect of orally ingested collagen peptides (CPs) on skin condition and elucidate their mechanism of action. Methods: A randomized, placebo-controlled, double-blind trial was conducted in 99 healthy Japanese women, aged 35–50 years. The subjects were randomized into 3 groups (33 subjects/group) to receive 1 or 5 g of CP or placebo once daily for 12 weeks. Skin water content, transepidermal water loss (TEWL), skin elasticity, and skin thickness were evaluated before treatment and after 4, 8, and 12 weeks of treatment. The level of natural moisturizing factor (NMF) constituents in the stratum corneum (SC) was quantified before treatment and after 12 weeks of treatment. Results: Oral ingestion of CP increased the water content in the SC and epidermis and decreased TEWL. Furthermore, the NMF level in the SC was increased. However, skin elasticity and skin thickness remained unchanged. Conclusions: The improvement in skin water content following the oral ingestion of CP can be attributed to an increase in the level of NMF in the SC. Trial Registration: UMIN000030375 (retrospectively registered).
References
1.
Leider
M
. On the weight of the skin
. J Invest Dermatol
. 1949 Mar
;12
(3
):187
–91
. .2.
Slominski
AT
, Manna
PR
, Tuckey
RC
. On the role of skin in the regulation of local and systemic steroidogenic activities
. Steroids
. 2015 Nov
;103
:72
–88
. .3.
Ohara
H
, Ito
K
, Iida
H
, Matsumoto
H
. Improvement in the moisture content of the stratum corneum following 4 weeks of collagen hydrolysate ingestion
. J Food Sci Technol Res
. 2009 Apr
;56
(3
):137
–45
. .4.
Choi
SY
, Ko
EJ
, Lee
YH
, Kim
BG
, Shin
HJ
, Seo
DB
, et al. Effects of collagen tripeptide supplement on skin properties: a prospective, randomized, controlled study
. J Cosmet Laser Ther
. 2014 Jun
;16
(3
):132
–7
. .5.
Sugihara
F
, Inoue
N
, Wang
X
. Clinical effects of ingesting collagen hydrolysate on facial skin properties. -A randomized, placebo-controlled, double-blind trial
. Jpn Pharmacol Ther
. 2015 Jan
;43
:67
–70
.6.
Proksch
E
, Segger
D
, Degwert
J
, Schunck
M
, Zague
V
, Oesser
S
. Oral supplementation of specific collagen peptides has beneficial effects on human skin physiology: a double-blind, placebo-controlled study
. Skin Pharmacol Physiol
. 2014 Aug
;27
(1
):47
–55
. .7.
Proksch
E
, Schunck
M
, Zague
V
, Segger
D
, Degwert
J
, Oesser
S
. Oral intake of specific bioactive collagen peptides reduces skin wrinkles and increases dermal matrix synthesis
. Skin Pharmacol Physiol
. 2014 Dec
;27
(3
):113
–9
. .8.
Miyanaga
M
, Uchiyama
T
, Ueda
O
. Oral intake of collagen peptide improves skin condition
. Pharmacometrics
. 2016 July
;90
:101
–7
.9.
Rawlings
AV
, Scott
IR
, Harding
CR
, Bowser
PA
. Stratum corneum moisturization at the molecular level
. J Invest Dermatol
. 1994 Jun
;103
(5
):731
–41
. .10.
Scott
IR
, Harding
CR
, Barrett
JG
. Histidine-rich protein of the keratohyalin granules. Source of the free amino acids, urocanic acid and pyrrolidone carboxylic acid in the stratum corneum
. Biochim Biophys Acta
. 1982 Oct
;719
(1
):110
–7
. .11.
Horii
I
, Kawasaki
K
, Koyama
J
, Nakayama
Y
, Nakajima
K
, Okazaki
K
, et al. Histidine-rich protein as a possible origin of free amino acids of stratum corneum
. J Dermatol
. 1983 Feb
;10
(1
):25
–33
. .12.
Szakall
A
. [Properties, origin and physiological functions of the substances of the keratinized epidermis determining the hydrogen-ion concentration]
. Arch Klin Exp Dermatol
. 1955 July
;201
(4
):331
–60
. .13.
Jacobi
OK
. About the mechanism of moisture regulation in horny layer of the skin
. Proc Sci Sect TGA
. 1959
;31
:22
–4
.14.
Sakai
S
, Yasuda
R
, Sayo
T
, Ishikawa
O
, Inoue
S
. Hyaluronan exists in the normal stratum corneum
. J Invest Dermatol
. 2000 Jun
;114
(6
):1184
–7
. .15.
Koyama
J
, Kawasaki
K
, Horii
I
, Nakayama
Y
, Morikawa
Y
. Relation between dry skin and water soluble components in the stratum corneum
. J Soc Cosmet Chem Japan
. 1983 Mar
;16
(2
):119
–24
. .16.
Horii
I
, Nakayama
Y
, Obata
M
, Tagami
H
. Stratum corneum hydration and amino acid content in xerotic skin
. Br J Dermatol
. 1989 Nov
;121
(5
):587
–92
. .17.
Takahashi
M
. Skin care in atopic dermatitis: characterizing dry skin in atopic dermatitis and utility of skin care
. J Jpn Cosmet Sci Soc
. 1997
;21
:50
–5
.18.
Tanaka
M
, Okada
M
, Zhen
YX
, Inamura
N
, Kitano
T
, Shirai
S
, et al. Decreased hydration state of the stratum corneum and reduced amino acid content of the skin surface in patients with seasonal allergic rhinitis
. Br J Dermatol
. 1998 Oct
;139
(4
):618
–21
. .19.
Denda
M
, Hori
J
, Koyama
J
, Yoshida
S
, Nanba
R
, Takahashi
M
, et al. Stratum corneum sphingolipids and free amino acids in experimentally-induced scaly skin
. Arch Dermatol Res
. 1992 Dec
;284
(6
):363
–7
. .20.
Uchiyama
T
, Miyanaga
M
, Ueda
O
, Ogo
M
. Oral intake of amula fruit and lingonberry extracts improves skin elasticity
. Pharmacometrics
. 2016
;91
:85
–91
.21.
Shimbo
K
, Yahashi
A
, Hirayama
K
, Nakazawa
M
, Miyano
H
. Multifunctional and highly sensitive precolumn reagents for amino acids in liquid chromatography/tandem mass spectrometry
. Anal Chem
. 2009 Jul
;81
(13
):5172
–9
. .22.
Ziff
M
, Kibrick
A
, Dresner
E
, Gribetz
HJ
. Excretion of hydroxyproline in patients with rheumatic and non-rheumatic diseases
. J Clin Invest
. 1956 Jun
;35
(6
):579
–87
. .23.
Prockop
DJ
, Sjoerdsma
A
. Significance of urinary hydroxyproline in man
. J Clin Invest
. 1961 May
;40
:843
–9
. .24.
Meilman
E
, Urivetzky
MM
, Rapoport
CM
. Urinary hydroxyproline peptides
. J Clin Invest
. 1963 Jan
;42
:40
–50
. .25.
Iwai
K
, Hasegawa
T
, Taguchi
Y
, Morimatsu
F
, Sato
K
, Nakamura
Y
, et al. Identification of food-derived collagen peptides in human blood after oral ingestion of gelatin hydrolysates
. J Agric Food Chem
. 2005 Aug
;53
(16
):6531
–6
. .26.
Yamamoto
S
, Deguchi
K
, Onuma
M
, Numata
N
, Sakai
Y
. Absorption and urinary excretion of peptides after collagen tripeptide ingestion in humans
. Biol Pharm Bull
. 2016 Mar
;39
(3
):428
–34
. .27.
Jimbo
N
, Kawada
C
, Nomura
Y
. Optimization of dose of collagen hydrolysate to prevent UVB-irradiated skin damage
. Biosci Biotechnol Biochem
. 2015 Sep
;80
(2
):356
–9
. .28.
Ohara
H
, Ichikawa
S
, Matsumoto
H
, Akiyama
M
, Fujimoto
N
, Kobayashi
T
, et al. Collagen-derived dipeptide, proline-hydroxyproline, stimulates cell proliferation and hyaluronic acid synthesis in cultured human dermal fibroblasts
. J Dermatol
. 2010 Apr
;37
(4
):330
–8
. .29.
Kang
M
, Yumnam
S
, Kim
S
. Oral intake of collagen peptide attenuates ultraviolet B irradiation-induced skin dehydration in vivo by regulating hyaluronic acid synthesis
. Int J Mol Sci
. 2018 Nov
;19
(11
):3551
. .30.
Torvard
CL
, Ulla
BGL
, J Robert
EF
. Functions of hyaluronan
. Ann Rheum Dis
. 1995 May
;54
:429
–32
.© 2021 S. Karger AG, Basel
2021
Copyright / Drug Dosage / Disclaimer
Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.
You do not currently have access to this content.
