Background/Aims: Our previous study confirmed that Aloe sterol stimulates collagen and hyaluronic acid production in human dermal fibroblasts. This study aims to investigate whether Aloe sterol intake affects skin conditions. Methods: We performed a 12-week, randomized, double-blind, placebo-controlled study to evaluate the effects of oral Aloe sterol supplementation on skin elasticity, hydration, and the collagen score in 64 healthy women (age range 30-59 years; average 44.3 years) who were randomly assigned to receive either a placebo or an Aloe sterol-supplemented yogurt. Skin parameters were measured and ultrasound analysis of the forearm was performed. Results: ANCOVA revealed statistical differences in skin moisture, transepidermal water loss, skin elasticity, and collagen score between the Aloe sterol and placebo groups. The gross elasticity (R2), net elasticity (R5), and biological elasticity (R7) scores of the Aloe sterol group significantly increased with time. In addition, skin fatigue area F3, which is known to decrease with age and fatigue, also increased with Aloe sterol intake. Ultrasound echogenicity revealed that the collagen content in the dermis increased with Aloe sterol intake. Conclusion: The results suggest that continued Aloe sterol ingestion contributes to maintaining healthy skin.

Keywords:
Aloe vera, Aloe sterol, Skin moisture, Skin elasticity, Collagen score
1.
Calleja-Agius J, Brincat M, Borg M: Skin connective tissue and ageing. Best Pract Res Clin Obstet Gynaecol 2013;27:727-740.
2.
Baumann L: Skin ageing and its treatment. J Pathol 2007;211:241-251.
3.
Liu X, Wu H, Byrne M, Krane S, Jaenisch R: Type III collagen is crucial for collagen I fibrillogenesis and for normal cardiovascular development. Proc Natl Acad Sci USA 1997;94:1852-1856.
4.
Varani J, Dame MK, Rittie L, Fligiel SE, Kang S, Fisher GJ, Voorhees JJ: Decreased collagen production in chronologically aged skin: roles of age-dependent alteration in fibroblast function and defective mechanical stimulation. Am J Pathol 2006;168:1861-1868.
5.
Farage MA, Miller KW, Berardesca E, Maibach HI: Clinical implications of aging skin: cutaneous disorders in the elderly. Am J Clin Dermatol 2009;10:73-86.
6.
Koyano Y, Nakagami G, Iizaka S, Minematsu T, Noguchi H, Tamai N, Mugita Y, Kitamura A, Tabata K, Abe M, Murayama R, Sugama J, Sanada H: Exploring the prevalence of skin tears and skin properties related to skin tears in elderly patients at a long-term medical facility in Japan. Int Wound J 2016;13:189-197.
7.
Shelton RM: Aloe vera: its chemical and therapeutic properties. Int J Dermatol 1991;30:679-683.
8.
Vogler BK, Ernst E: Aloe vera: a systematic review of its clinical effectiveness. Br J Gen Pract 1999;49:823-828.
9.
Tanaka M, Misawa E, Ito Y, Habara N, Nomaguchi K, Yamada M, Toida T, Hayasawa H, Takase M, Inagaki M, Higuchi R: Identification of five phytosterols from Aloe vera gel as anti-diabetic compounds. Biol Pharm Bull 2006;29:1418-1422.
10.
Nomaguchi K, Tanaka M, Misawa E, Yamada M, Toida T, Iwatsuki K, Goto T, Kawada T: Aloe vera phytosterols act as ligands for PPAR and improve the expression levels of PPAR target genes in the livers of mice with diet-induced obesity. Obes Res Clin Pract 2011;5:190-201.
11.
Chinetti G, Fruchart JC, Staels B: Peroxisome proliferator-activated receptors (PPARs): nuclear receptors at the crossroads between lipid metabolism and inflammation. Inflamm Res 2000;49:497-505.
12.
Barlaka E, Görbe A, Gáspár R, Pálóczi J, Ferdinandy P, Lazou A: Activation of PPARβ/δ protects cardiac myocytes from oxidative stress-induced apoptosis by suppressing generation of reactive oxygen/nitrogen species and expression of matrix metalloproteinases. Pharmacol Res 2015;95-96:102-110.
13.
Saito M, Tanaka M, Misawa E, Yao R, Nabeshima K, Yamauchi K, Abe F, Yamamoto Y, Furukawa F: Oral administration of Aloe vera gel powder prevents UVB-induced decrease in skin elasticity via suppression of overexpression of MMPs in hairless mice. Biosci Biotechnol Biochem 2016;80:1416-1424.
14.
Xia Q, Yin JJ, Fu PP, Boudreau MD: Photo-irradiation of Aloe vera by UVA - formation of free radicals, singlet oxygen, superoxide, and induction of lipid peroxidation. Toxicol Lett 2007;168:165-175.
15.
Chithra P, Sajithlal GB, Chandrakasan G: Influence of Aloe vera on the healing of dermal wounds in diabetic rats. J Ethnopharmacol 1998;59:195-201.
16.
Oryan AT, Naeini A, Nikahval B, Gorjia E: Effect of aqueous extract of Aloe vera on experimental cutaneous wound healing in rat. Vet Arch 2010;80:509-522.
17.
Tanaka M, Misawa E, Yamauchi K, Abe F, Ishizaki C: Effects of plant sterols derived from Aloe vera gel on human dermal fibroblasts in vitro and on skin condition in Japanese women. Clin Cosmet Investig Dermatol 2015;895-104.
18.
Cho S, Lee S, Lee M-J, Lee DH, Won C-H, Kim SM, Chung JH: Dietary Aloe vera supplementation improves facial wrinkles and elasticity and it increases the type 1 procollagen gene expression in human skin in vivo. Ann Dermatol 2009;21:6-11.
19.
Tzellos TG, Klagas I, Vahtsevanos K, Triaridis S, Printza A, Kyrgidis A, Karakiulakis G, Zouboulis CC, Papakonstantinou E: Extrinsic ageing in the human skin is associated with alterations in the expression of hyaluronic acid and its metabolizing enzymes. Exp Dermatol 2009;18:1028-1035.
20.
Lodén M, Olsson H, Axéll T, Linde YW: Friction, capacitance and transepidermal water loss (TEWL) in dry atopic and normal skin. Br J Dermatol 1992;126:137-141.
21.
Hashizume H: Skin aging and dry skin. J Dermatol 2004;31:603-609.
22.
Takema Y, Yorimoto Y, Kawai M, Imokawa G: Age-related changes in the elastic properties and thickness of human facial skin. Br J Dermatol 1994;131:641-648.
23.
Dobrev H: Use of Cutometer to assess dermal oedema in erysipelas of the lower legs. Skin Res Technol 1998;4:155-159.
24.
Dobrev H: A study of human skin mechanical properties by means of Cutometer. Folia Med 2002;44:5-10.
25.
Ryu HS, Joo YH, Kim SO, Park KC, Youn SW: Influence of age and regional differences on skin elasticity as measured by the Cutometer. Skin Res Technol 2008;14:354-358.
26.
Dobrev H: Application of Cutometer area parameters for the study of human skin fatigue. Skin Res Technol 2005;11:120-122.
27.
Ohshima H, Kinoshita S, Oyobikawa M, Futagawa M, Takiwaki H, Ishiko A, Kanto H: Use of Cutometer area parameters in evaluating age-related changes in the skin elasticity of the cheek. Skin Res Technol 2013;19:e238-e242.
28.
Gniadecka M, Jemec GB: Quantitative evaluation of chronological ageing and photoageing in vivo: studies on skin echogenicity and thickness. Br J Dermatol 1998;139:815-821.
29.
Rigal J, Escoffier C, Querleux B, Faivre B, Agache P, Lévêque JL: Assessment of aging of the human skin by in vivo ultrasonic imaging. J Invest Dermatol 1989;93:621-625.
30.
Sandby-Møller J, Wulf HC: Ultrasonographic subepidermal low-echogenic band, dependence of age and body site. Skin Res Technol 2004;10:57-63.
31.
Wulf HC, Poulsen T, Davies RE, Urbach F: Narrow-band UV radiation and induction of dermal elastosis and skin cancer. Photodermatol 1989;6:44-51.
32.
Gilchrest BA, Yaar M: Ageing and photoageing of the skin observations at the cellular and molecular level. Br J Dermatol 1992;127:25-30.
33.
Fligiel SE, Varani J, Datta SC, Kang S, Fisher GJ, Voorhees JJ: Collagen degradation in aged/photodamaged skin in vivo and after exposure to matrix metalloproteinase-1 in vitro. J Invest Dermatol 2003;120:842-848.
34.
Ishikawa F, Inoue N, Ikeda I, Tanaka M, Yamada M: Internal accumulation of the plant sterol derived from the aloe in the rat. 49th Annu Meet Jpn Oil Chem Soc, Hakodate, 2010, p 180.
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