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Keywords: Reporter gene assay
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Journal Articles

Adenoviral E1A Suppresses Secretory Leukoprotease Inhibitor and Elafin Secretion in Human Alveolar Epithelial Cells and Bronchial Epithelial Cells

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Subject Area:
Pharmacology , Pneumology
Yuji Higashimoto, Yuko Yamagata, Takuya Iwata, Tadashi Ishiguchi, Morihiro Okada, Mitsunori Masuda, Hiroaki Satoh, Hidekazu Itoh
Journal: Respiration
Respiration (2005) 72 (6): 629–635.
https://doi.org/10.1159/000089579
Published Online: 08 December 2005
... using an ELISA. SLPI mRNA expression was examined by Northern blotting, and SLPI promoter activity was measured using a reporter gene assay. Results: E1A significantly suppressed SLPI and elafin/SKALP secretion by A549 cells upon interleukin (IL)-1β stimulation. E1A also suppressed SLPI and elafin/SKALP...
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