... sputum Chronic obstructive pulmonary disease Dithiothreitol Transcriptome Induced sputum is currently the best available noninvasive assessment of airway inflammation in chronic airway diseases such as asthma and chronic obstructive pulmonary disease (COPD). Accordingly, whole-genome gene...

... remains controversial. Objectives: The aim of this study was to compare the concentration of IL-6 and IL-13 in the induced sputum of asthma and COPD patients, and to assess the possible association of these cytokines with the impairment of lung function. Methods: Twenty-six subjects with COPD and 18...

... but variable airflow limitation indicates that bronchial constriction is involved in the mechanism of cough in CVA [ 3 ]. Cough receptor sensitivity Cough variant asthma Leukotriene receptor antagonist Montelukast Induced sputum Cysteinyl leukotrienes Eosinophils Findings in induced sputum...

... = 0.042) and showed a greater number of macrophages and neutrophils in induced sputum (p = 0.020 and p < 0.001, respectively) than those with asthma. Table 2 Characteristics and findings of patients with asthma and their age-matched controls Table 3 Characteristics and findings...

... for interstitial lung diseases. Serum levels of SP-A and SP-D have also been found to be elevated in chronic obstructive pulmonary disease (COPD), but their significance has not been evaluated or compared in induced sputum samples obtained directly from the airways. Objective: A sequential sputum analysis...

...A. Hector; F. Jonas; M. Kappler; M. Feilcke; D. Hartl; M. Griese Background: Induced sputum is the most commonly used method to analyze airway inflammation in cystic fibrosis (CF) patients ex vivo. Due to the complex matrix of the sample material, precise and reliable analysis of sputum...

...Jodie L. Simpson; Patrick McElduff; Peter G. Gibson Background: The assessment of eosinophilic airway inflammation using induced sputum identifies a corticosteroid-responsive subtype that can be used to guide anti-inflammatory therapy. The stability of airway inflammation in asthma over time...

... , NO, CO, and O 3 produced during the SIC did not exceed threshold limit values. Samples of induced sputum were obtained before and after the SIC and showed an increase in neutrophils and concentrations of IL-8, TNF-α and TNF-β after the SIC. This study presents the first clinical findings reported...

...Fei Wang; Bei He Background: Induced sputum analysis has been widely used to assay airway inflammation. However, the mucolytic agent dithiothreitol (DTT) may affect the detection of some soluble mediators. Objectives: Our study was designed to investigate whether DTT affects the detection...

... these mediators and airflow limitation in AECOPD patients. Methods: TNF-α, sTNF-R55 and sTNF-R75 levels in induced sputum and plasma as well as lung functions were examined in 48 AECOPD patients before and after treatment. Samples from 28 healthy volunteers served as controls. Results: Compared to healthycontrols...

... in noninvasive specimens such as exhaled breath condensate (EBC) and induced sputum in healthy nonsymptomatic smokers or in those who have symptoms and are at risk for COPD development. Objectives: To compare the relative proportions of 2 potential COPD biomarkers, 8-isoprostane and interleukin- 8 (IL-8...

... bronchitis and patients with pulmonary emphysema. Conclusions: The pattern of airway inflammation in induced sputum of patients with chronic asthma is different from that of COPD patients with a similar FEV 1 . Among COPD patients, however, the pattern of airway inflammation shows no difference between...

...Foteini Economidou; Katerina D. Samara; Katerina M. Antoniou; Nikolaos M. Siafakas The search for noninvasive procedures of retrieving cells and soluble material from the lung has gained momentum over the past few years. Induced sputum (IS) by inhalation of hypertonic saline solution...

...N. Damjanov; P. Ostojic; O. Kaloudi; S. Alari; S. Guiducci; N. Stanflin; B. Nestorovic; J. Knezevic; G. Camiciottoli; F. Porta; M. Pistolesi; L. Ibba-Manneschi; M.L. Conforti; A. Candelieri; M. Matucci Cerinic Background: Induced sputum (IS) is a noninvasive tool, which can be used to collect...

... was to confirm the effect of a short-term treatment with oral glucocorticosteroids on O 3 -induced airway inflammation, detected by induced sputum analysis, and on functional response in glucocorticosteroid-naive subjects. Methods: A randomized, placebo-controlled study using oral prednisone (25 mg o.d. for 4...

... non-responders). T-lymphocyte subsets in induced sputum were analyzed before and 1 year after IFN therapy. Results: Before IFN therapy, percentages of lymphocytes and CD8+ T lymphocytes in induced sputum were higher in HCV-positive asthmatics than in HCV-negative asthmatics. After IFN therapy...

...- and post-treatment values and those of sputum PICP concentrations between spontaneous and induced sputum samples were tested using a paired t test. A comparison among the three groups was performed using the Kruskal-Wallis test. Linear regression analysis was conducted using the Pearson correlation test. p...

...Diahn-Warng Perng; Hsuan-Tsung Su; Chih-Wei Tseng; Yu-Chin Lee We report a case of pulmonary infiltrates with eosinophilia (PIE) induced by nimesulide in an asthmatic patient on regular inhaled corticosteroid treatment. A high level of eosinophils in induced sputum was detected. No evidence...

...Katerina M. Antoniou; Michaels Alexandrakis; Nikolaos Tzanakis; Ioanna Tsiligianni; Eleni G. Tzortzaki; Nikolaos M. Siafakas; Demosthenes E. Bouros Background: Induced sputum (IS) has been proposed as a useful noninvasive method for the assessment of airway diseases. Bronchoalveolar lavage fluid...

... exposure activates the inflammatory/repair process in sensitized subjects. Induced-sputum analysis is a non-invasive method that allows the assessment of changes in inflammatory and remodeling mediators implicated in asthma. Objectives: To evaluate the changes in MMP-9 and its principal inhibitor (TIMP-1...