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Keywords: Reporter gene assay
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Journal Articles
Validation of a HeLa Mx2 /Luc Reporter Cell Line for the Quantification of Human Type I Interferons
Available to PurchaseSubject Area:
Pharmacology
Young-Jun Seo, Gi-Hyun Kim, Ho-Jung Kwak, Ju-Sun Nam, Hwa Jeong Lee, Soo-Kyung Suh, Kyung-Min Baek, Yeo-won Sohn, Seung-Hwa Hong
Journal:
Pharmacology
Pharmacology (2009) 84 (3): 135–144.
Published Online: 14 August 2009
... of an effect of the HeLa- Mx2 cell culture’s age on the performance of the reporter gene assay. The reporter gene assay demonstrated reproducible dose-response curves for IFN-α2a in the range of 1–10,000 IU/ml. The 95% confidential limit and total coefficient of variation estimates ranged between 96 and 116...
Journal Articles
Leukotriene D 4 Upregulates MUC2 Gene Transcription in Human Epithelial Cells
Available to PurchaseSubject Area:
Pharmacology
Journal:
Pharmacology
Pharmacology (2008) 81 (3): 221–228.
Published Online: 07 January 2008
... is known about the effect of LTD 4 on mucin gene expression. The aim of this study was to investigate the effect of LTD 4 on MUC2 gene expression in cultured epithelial cells (HM3- MUC2 cells). Methods: HM3- MUC2 cells were treated with LTD 4 for 2 or 6 h. Reporter gene assay was mainly used...
