.... Functionally, AGAP2-AS1 regulated trastuzumab resistance by inducing autophagy and increasing ATG5 expression. Conclusion: we demonstrated that METTL3/YTHDF2-mediated m6A methylation increased the expression of AGAP2-AS1, which could promote trastuzumab resistance in breast cancer. AGAP2-AS1 regulates...

..., improved heart rate variability, reduced ventricular arrhythmia score, and ameliorated cardiac dysfunction in IR rats. Furthermore, intrathecal bupivacaine attenuated cardiac injury and myocardial apoptosis and regulated cardiomyocyte autophagy and connexin-43 distribution during myocardial IR. Conclusion...

... cells, including breast, lung, and liver cancers. The anticancer properties of sinomenine are believed to involve stimulation of apoptosis and autophagy as well as suppression of cell proliferation, invasion, and metastasis. Key Message: This review summarizes the current research on sinomenine’s...

...Yan Lu; Hanlin Zhang; Min Han; Ping Wang; Liping Meng Introduction: Hyperuricemia may be involved in the phenotypic transformation of vascular smooth muscle cells, thus promoting the occurrence of atherosclerosis, and autophagy may be one of the important links, but little is known about...

... of osteoarthritic cartilage. PG545 is a heparan sulfate mimetic with heparanase inhibitory activity. In this study, the therapeutic effects and possible mechanisms of PG545 were investigated in a chondrocyte injury model induced by interleukin-1β (IL -1β). Methods: Following treatment with PG545 or the autophagy...

...Ting Zhang; Jia Yu; Sha Cheng; Ya Zhang; Chang-hua Zhou; Juan Qin; Heng Luo Background: Autophagy is a lysosome-mediated catabolic process that maintains cell homeostasis and survival. It occurs not only in normal cells such as cardiac muscle cells, neurons, and pancreatic acinar cells but also...

... estimated via MTT and colony formation experiments. Flow cytometry analysis was adopted to evaluate the apoptosis rate of HCT-116 and LoVo cells. Apoptosis-related proteins (Bcl-2, Bax, and caspase-3) and autophagy-related proteins (LC3 and P62) were assessed by Western blotting. Effects of Rau on autophagy...

... exerts cardioprotection against I/R- or OGD/R-induced injury, and its mechanisms may be associated with the reduction in RIP1K activation and restoration impaired autophagic flux. DIMO Myocardial infarction Necroptosis Autophagy Cardioprotection Ischemic heart disease, especially acute...

...Youli Tao; Hua Zhou; Lili Huang; Xiaoyin Xu; Yun Huang; Lili Ma; Lingna Li; Xu Yao; Ronghui Zhang; Yuyu Zhang; Weibo Rong; Chaojun Yang; Taotao Yang; Yi Shen; Rixiang Wang Introduction: Although oxidative stress has been demonstrated to mediate acute ethanol-induced changes in autophagy...

... the overexpression of TRPV6 or the inhibition of JNK signalling markedly enhanced cell viability, inhibited apoptosis, and autophagy and reduced p-JNK levels in TSA-treated cells. The inhibition of STC1 significantly increased TRPV6 protein levels and reduced p-JNK levels. Overexpression of PRMT5 dramatically...

...Zhonglei Bao; Huixue Zhang; Hong Jiao; Xiaojie Chu; Jin Fu; Lihua Wang Introduction: The plaques formed by amyloid-β (Aβ) accumulation and neurofibrillary tangles formed by hyper-phosphorylated tau protein are the 2 major pathologies of Alzheimer’s disease (AD). Recently, autophagy is considered...

... cell model and whether autophagy is involved in the therapeutic function of astragaloside IV. Methods: MLE-12 cells were induced by LPS to construct an ARDS model in vitro. Cell viability was estimated by cell counting kit-8 and cell apoptosis by flow cytometry. Lactate dehydrogenase (LDH...

... by studying its effects on autophagy, vesicle formation, and lysosomal organisation. Summary: Vesicle formation and autophagy induction were analysed by transmission electron microscopy (TEM), monodansylcadaverine (MDC) staining and Lysotracker staining, while autophagosome turnover was analysed using...

..., cycle, apoptosis, and autophagy of human glioma cell U251 by regulating phosphatidylinositol 3-kinase (PI3K)/Akt/mammalian target of rapamycin (mTOR) signal pathway. Methods: Tan IIA and PI3K agonist (740 Y-P) were used to treat glioma cells U251. MTT assay was used to assess cell viability and flow...

..., the detailed mechanisms of the preventive effects have not been clarified. We examined the relationship between the preventive effects of HKT and autophagy, a known stress response and quality control mechanism, using Madin-Darby canine kidney cells and influenza A/PR/8/34 (H1N1) virus. Methods: The effect...

... distribution, mitochondrial membrane potential and annexin V staining were analyzed using flow cytometry. Western blotting was used to determine the levels of apoptosis- and autophagy-related proteins. Results: CuB inhibited the proliferation of Jurkat cells in a dose-dependent manner and induced G 2 /M phase...

...Min Wen; Jun Wu; Hui Luo; Haitao Zhang Aim: To investigate the mechanism by which galangin, a polyphenolic compound derived from medicinal herbs, induces autophagy of HepG2 cells. Methods: The MTT [3-(4,5-dimethyl-thiazol-2-yl)2,5-diphenyl-tetrazolium bromide] assay was used to measure cell...