... by Western blot analysis. The receptor coupling efficiency and biochemical activities of the adenylate cyclase (AC) was also determined. Results: In HCC liver membranes, the β 2 -AR density was higher than the density in the nonadjacent nontumor liver membranes. The β 2 -AR protein expression was 1.5-fold...

... trend in adenylate cyclase VI expression; however, STS supplementation reversed these effects in AVF mice. Among 2 enzymes that produce endogenous hydrogen sulfide (H 2 S), cystathionine-γ-lyase (CSE) expression was attenuated in AVF mice with no changes in cystathionine-β-synthase (CBS) expression...

...R. Reimer; H.S. Odes; W. Beil; M. Schwenk; R. Muallem; K.-F. Sewing To get information about the peptide hormone receptors involved in duodenal bicarbonate secretion (DBS) and their cellular location, we determined DBS and adenylate cyclase (AC) activity in response to hormones of the vasoactive...

..., and of the binding of [ 3 ]N-ethylcarboxamidoadenosine to A 2 adenosine receptors in rat striatal membranes and as agonists at A 2 adenosine receptors coupled to adenylate cyclase in rat pheochromocytoma PC 12 cell membranes. The activities are consonant with a hydrophobic binding site in the A 2 recpetors...

...Walter Trizna; Richard M. Edwards The effects of parathyroid hormone (PTH) and parathyroid hormone-related protein (PTHrP) on renal arteriolar tone and proximal tubule adenylate cyclase were examined. In both afferent and efferent arterioles, PTH produced concentration-dependent relaxation...

...W. Beil; H. Hannemann; K.-F. Sewing The tricyclic antidepressants trimipramine and doxepin, and the neuroleptic agents trifluoperazine and haloperidol were tested for their effect on histamine H 2 -receptor-mediated adenylate cyclase activity and H + secretion in guinea-pig parietal cells. All...

...W. Beil; R.J. Bersimbaev; H Hannemann; K.-F. Sewing The calcium channel antagonists verapamil, gallopamil and nifedipine were tested for their effects on acid secretion stimulated by histamine and dibutyryl-cyclic AMP in isolated and enriched guinea pig parietal cells, on adenylate cyclase activity...

...M. Becker; S.E. Miederer; H.-J. Ruoff Activation of adenylate cyclase (AC) by PGE 2 , histamine and gastrointestinal hormones was studied in parietal cell-free gastric biopsy specimens from the corpus of patients with proven high gastrin achlorhydria. PGE 2 , somatostatinVIP, pentagastrin...

...H.-J. Ruoff; T. Leyhe; G. Pfaff; G. Rettenmaier; E. Schmid Adenylate cyclase (AC) in response to prostaglandin E 2 (PGE 2 ) and histamine was studied in morphologically different biopsy specimens from human gastric mucosa. The activities of the enzyme were log-normally distributed and did...

...A.M. Cheret; C. Scarpignato; M.J.M. Lewin; G. Bertaccini The effect of cimetidine and two new histamine H 2 -receptor antagonists, oxmetidine and SKF 93479, on histamine-stimulated adenylate cyclase activity was studied in guinea pig gastric mucosal cells. Histamine stimulated the enzyme activity...

...E. Söchtig; T. Trost Eritadenine was investigated for its effects on adenylate cyclase activity of rat fat cell plasma membranes and on cyclic AMP accumulation and lipolysis in isolated rat fat cells. In rat fat cell plasma membranes, a 50% inhibition of noradrenaline (100 µmol/l) stimulated...

...H.-J. Ruoff; Brigitte Painz; Ulrich Gladziwa; Michael Becker; K.-F. Sewing; Helmut Malchow Basal and adrenaline-stimulated adenylate cyclase (AC) was studied in biopsy specimens of the gastric and duodenal mucosa from 112 individuals. AC activities were log normally distributed. AC of fundic...

...Ted H. Chiu The activity of myocardial adenylate cyclase from two age groups (10–11 and 18–19 weeks old, respectively) of male spontaneously hypertensive rats (SHR) and their age-matched normotensive Wistar-Kyoto rats (WKY) was examined. Stimulation of the enzyme by isoproterenol and 5’-guanylyl...

...Gene C. Palmer; Stan Greenberg Particulate fractions of myocardium taken from spontaneously hypertensive rats (SHR) contained an adenylate cyclase system that was less responsive than normotensive Wistar Kyoto rats to norepinephrine, isoproterenol (mixed β-agonist), salbutamol (β 2 -agonist), do...

... cyclic AMP. Adenylate cyclase activity was increased by addition of plasma into the incubation medium. However, splenic venous plasma obtained during splenic nerve stimulation did not increase adenylate cyclase activity more than control plasma obtained before stimulation. We conclude that the positive...

...P. Portaleone We compared the effects of bromocriptine and of hydergine on striatal and hypothalamic adenylate cyclase activity. Rats treated with bromocriptine (5 mg/kg) or with hydergine (3 mg/kg), showed a stimulation of adenylate cyclase activity in the striatum, but not in the hypothalamus...

...Aleksander A. Mathé; Surendra K. Puri; Ladislav Volicer; Richard J. Sohn Cyclic AMP levels and adenylate cyclase and phosphodiesterase activities were measured in control and ovalbumin-sensitized guinea pig lungs. Cyclic AMP levels were raised by epinephrine (0.01–10 μg/ml) in both control...

...L. Volicer; P. Polgar; S.L.N. Rao; A.M. Rutenburg The wall of the bovine carotid artery was divided into three layers, two consisting largely of smooth muscle and one containing mainly connective tissue. Cyclic AMP and ATP levels as well as the activities of adenylate cyclase and phosphodiesterase...