Wood creosote, a mixture of phenolic compounds, inhibits enterotoxin-induced intestinal fluid secretion, suggesting that one of its constituents suppresses transepithelial Cl– secretion from the intestinal mucosa. To identify an active constituent in wood creosote that inhibits intestinal Cl– secretion through Cl– channels, we first examined its effect on Cl– secretion using a cultured cell line transfected with complementary DNA encoding a Cl–channel and a Cl–-sensitive fluorescent dye. We next assayed chromatographic fractions of wood creosote for the inhibitory activity on Cl– secretion using a Ussing chamber. We found that 4,5-dimethylresorcinol, identified by gas chromatography-mass spectrometry, inhibited intestinal Cl– secretion dose-dependently when added to a serosal, but not mucosal, surface of rat jejunum, a half-inhibitory concentration being 3.8 µg/ml (28 µmol/l). It was strongly suggested that this effect was due to inhibition of Cl– channels.

Keywords:
Cystic fibrosis membrane conductance regulator, Chloride ion, Intestine, Jejunum, Secretion, Ion channel, Rat, 4,5-Dimethylresorcinol, Ussing chamber, Inhibition
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© 2004 S. Karger AG, Basel
2004
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