A simple, sensitive, radioisotopic method to measure the formation of adenosine 3’,5’-cyclic monophosphate (cyclic AMP) by brain slices is described and evidence for its specificity is given. The method was used to study the effect of several drugs and hormones on the accumulation of cyclic AMP in slices from several regions of the brain of different animal species. The marked T stimulatory effect of histamine on the formation of cyclic AMP in rabbit and guinea pig brain cortex is peculiar to these species and does not occur in the brain cortex of rat, mouse, cat and monkey. In the cortex of these last species, norepinephrine (NE) has been found to be the most active agent in increasing cyclic AMP formation. Marked differences between brain regions of different animal species were also found. Although theophylline increases cyclic AMP in various other tissue preparations, it does not enhance the histamine or NE induced formation of cyclic AMP in incubated brain slices.

Copyright / Drug Dosage / Disclaimer
Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.
You do not currently have access to this content.