The membrane ATP-ase was prepared from mucosa of human small intestine with differential centrifugation and treated with 2 M NaJ. The effect of butyl-biguanide, which inhibits the active transport (absorption) of glucose from the small intestine in man and also in animals, has been studied in relation to Mg++-dependent, total (Na+-K+-dependent and Mg++-dependent) and Na+-K+-dependent ATP-ase activities exclusively. A significant inhibition of total and Na+-K+-dependent ATP-ase was caused by butyl-biguanide, while no significant stimulation of Mg++-dependent ATP-ase activity was obtained. The possible role of Na+-K+-dependent ATP-ase activity in the glucose absorption from small intestine, on the one hand, and the inhibition by butyl-biguanide of glucose absorption, on the other hand, have been discussed.

Keywords:
Active transport, Glucose absorption, Human small intestine, Na+-K+-dependent ATP-ase
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© 1974 S. Karger AG, Basel
1974
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