... alterations using next-generation sequencing (NGS) in the non-pCR tumors is important, in order to identify potential opportunities of treatment for the patients, if targeted therapies are available. Methods: Breast cancer tissue samples of 31 patients, collected before NAT, were analyzed by NGS using...

... increasingly incorporate these findings in diagnostic algorithms. Therefore, clinical laboratories require versatile tools, which can detect an increasing number and variety of molecular and cytogenetic alterations of clinical significance. Methods: We validated an RNA-based next-generation sequencing (NGS...

...Josep Gumà; Karla Beatríz Peña; Francesc Riu; Clara Lucia-Gozálvez; Ana Vidaller; Vanesa Maldonado; David Parada Introduction: Liquid biopsy is an innovative and efficient method for studying circulating tumor DNA. In conjunction with innovative techniques such as next-generation sequencing, it can...

... or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. 2022 Tissue fixation Acid-deprived formalin DNA integrity Next-generation sequencing Archival, formalin-fixed, and paraffin-embedded (FFPE) tissue samples represent a rich...

...-tropomyosin receptor kinase Neurotrophic tropomyosin-related kinase Immunohistochemistry Gene fusion Next-generation sequencing The neurotrophic tropomyosin-related kinase ( NTRK ) gene family ( NTRK1 , NTRK2 , and NTRK3 ) encodes the tropomyosin receptor kinases (TRKs) A, B, and C (TRKA, TRKB...

... and 3 (60%) patients with sarcomatoid MPM. We found a TERT promoter mutation in 11.6% of MM, and the c.-146C>T mutation was the most common event. The next-generation sequencing was successful in 33 cases. A total of 18 samples showed at least 1 pathogenic, with a median of 1.8 variants, ranging from...

... at our institute of pathology. Methods: Our cohort comprised 60 primary and 8 metastatic GIST patients; 43 and 57% of the cases, respectively, were analyzed by Sanger sequencing or next-generation sequencing (NGS). Results: Of the 60 primary GIST patients, 47 (78%) showed a KIT mutation; 2 cases showed...

..., methods, instructions or products referred to in the content or advertisements. Bone marrow Workshop Myelodysplastic syndrome Next-generation sequencing Classification Cytopenia The diagnostic evaluation of patients with cytopenia requires the combination of clinical, laboratory...

... of a limited number of biomarkers. Recent developments in sequencing technology mean that DNA and RNA from tissue samples can now be interrogated in great detail. These new technologies, collectively known as next-generation sequencing (NGS), generate huge amounts of data which can be used to support patient...

...Eline van Winkel; Wendy W.J. de Leng; Petronella O. Witteveen; Trudy Jonges; Stefan M. Willems; Marlies H.G. Langenberg Objective: To investigate whether next-generation sequencing (NGS) in ovarian and endometrial tumours can discover mutations with a relevant prognostic or predictive value...

... the diagnosis, to guide treatment, to provide information about prognosis, and to allow patient recruitment for basket trials based on the molecular signature of a tumor. In addition, novel molecular alterations detected by next-generation sequencing (NGS) obtain further insights into the pathogenesis...

...Albrecht Stenzinger; Wilko Weichert Massive parallel sequencing technologies (next-generation sequencing) have enabled us to draw a comprehensive landscape of the genomic aberrations underlying common cancers of the digestive system, and they have thus revolutionized our understanding...