...Grzegorz Młynarczyk; Jacek Kudelski; Barbara Darewicz; Marta Bruczko-Goralewska; Lech Romanowicz Background: Collagenases are enzymes starting collagen degradation. The role of collagenases in renal carcinoma development is not well understood. Objective: Evaluation of collagen content...

...Edward Bańkowski; Krzysztof Sobolewski; Shirley Ayad; Suresh Gadher; David E. Woolley During conventional procedures for the extraction of collagen from rat fibrosarcoma it was observed that a significant fraction of the acid-soluble collagen remained insoluble when subsequently fractionated...

... was intended as a model to study the mechanisms of endothelial regeneration. All components were coated onto sterile coverslips at a concentration of 10 µg/ml. The highest expansion was obtained on fibronectin, laminin and collagen type III, all three being statistically significantly greater than...

...Thomas F. Kresina T cell hybridoma cell lines were generated by somatic cell fusion of BW 5147 myeloma cells and splenic cells from mice suppressed for collagen-induced arthritis (CIA). Two cell lines were characterized for their cell surface phenotype, antigen recognition and capacity to down...

... ECs (BCECs; 5 × 10 4 /ml) were used to investigate the adhesion of EC to collagen type IV and a mixture of fragments of the tetrameric molecule (IV-F, consisting of 75, 120 and 140 kD fragments), as well as collagen types I and III, coated at a 10-µg/ml concentration onto glass coverslips in vitro...

..., while MAB 29 and 52 bind to the same site. Antigenic fragments were identified by immunostaining of fibronectin tryptic peptides. MAB 5 reacted with a collagen binding fragment with a molecular weight of 120 K. In addition, each of the MAB 29, 30, 52 and 59 reacted with peptides with a molecular weight...

...Mireya Fernandez; Cristina Barahona; Jorge Martinez; Jose J. Minguell Bone marrow fibroblasts from normal and leukemic patients were used to investigate the relationship between serial subcultivation and changes in collagen synthesis. A regime was established to generate subcultures up to 35...

...Natsuo Yasui; Suguru Osawa; Takahiro Ochi; Hiroshi Nakashima; Keiro Ono Chondrocytes isolated from sternal cartilage of 13-day embryonic chicks were dispersively embedded inside the collagen gels in Ham’s F-12 medium containing fetal calf serum. Viability, three-dimensional growth and deposition...

... as internal controls. The data clearly indicated that an insufficient synthesis of type I collagen rather than a defect in the formation of cross-links may be the prime cause in this inherited connective tissue disorder. Such a disturbance in the regulation of the genes coding for the different collagen...

...Tomasz Guszczyn; Krzysztof Sobolewski Objective: The defects of collagen metabolism are responsible for the disorganization of extracellular matrix in stomach cancer. Collagen through interaction with integrin receptors regulates the cellular growth, differentiation, gene expression, prolidase...

... of both fibronectin and type III collagen. Fibronectin was seen in both early and latter responses to infarction while type III collagen was seen mainly in areas of incomplete healing among myocytes and around intramyocardial branches of the coronary arteries. The long-term low-dosage administration...