To examine the mechanism of induction of differentiation in the human malignant T-lymphoblastic cell line, MOLT-3, by 12-O-tetradecanoylphorbol-13-acetate (TPA), the role of receptors for phorbol esters was investigated. Binding of [20–3H]-phorbol-12,13-dibutyrate to TPA-resistant subclones derived from MOLT-3 was less than50 % of that of the parental MOLT-3. Scatchard analysis showed that the concentration of phorbol ester receptors in a TPA-resistant subclone was about 50% of that in the parental MOLT-3, but affinities of binding were similar, indicating that more than a certain number of phorbol ester receptors is required to induce differentiation by TPA in this human T cell line.

Keywords:
Phorbol ester receptor, Differentiation, MOLT-3 cells
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© 1988 S. Karger AG, Basel
1988
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