Soluble polyadenylic acid (poly(A)) polymerase content of stationary and growing cell populations from a variety (6) of cell lines was determined. Cell populations from stationary cultures presented poly(A) polymerase values with a mean of 31 ± 12 enzyme units/mg protein. The mean value for growing cell populations were 62 ± 18 enzyme units per mg protein. A statistically significant difference was found between stationary and growing cell populations from the variety of cell lines examined (p < 0.1). The observed differences in poly(A) polymerase levels persisted after fractionation of the crude extracts and revealed two molecular forms of enzyme activity with a net charge difference in stationary and growing cell cultures.

Keywords:
Stationary, Growing cells, Polyadenylate polymerase activity
© 1987 S. Karger AG, Basel
1987
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