... are specific to lens fibre cells and are therefore excellent markers for fibre cell differentiation. In the adult lens, filensin and CP49 are maintained throughout all stages of lens fibre cell differentiation whilst vimentin is apparently lost at a specific stage from the deeper cortical fibres...

...Frederick A. Bettelheim; Samuel Zigler Jr. Rat lenses stored for extended periods at -20°C showed degradation and eventual disappearance of vimentin. This may have been caused by the rupture of cells and/or organelles by growing ice crystals and the subsequent release and diffusion of proteases...

...Sharwan K. Kakar; Frederick A. Bettelheim The hydration of vimentin isolated from the cortex of bovine lenses was studied by thermal analysis. Differential scanning calorimetry between ––30, and 30°C provided the freezable water content, and thermogravimetric analysis up to 105°C yielded the total...

...Gregor Wollensak 20 experimental amelanotic Greene melanomas implanted into the anterior chamber of rabbit eyes were examined immunohistochemically in paraffin sections. All the Greene melanomas were found to be positive for vimentin, S-100 and HMB 45 and negative for α-human chorionic gonadotropin...

... to polyvinylidene fluoride membranes and probed with monoclonal antibodies to vimentin or cytokeratins, or a polyclonal antibody to phakinin. The monoclonal antibody to vimentin recognized the expected protein (M r = 56 kD, pI = 5.1) and several smaller and more acidic peptides including a 44-kD spot with a pI...

... we first separated the water-soluble fraction into the major crystallin classes by gel filtration. Moreover, we immunoblotted the gel of the urea-soluble fraction with a specific antibody against the intermediate filament protein vimentin. Upon irradiation of young and adult lenses, αA-crystallin...