... activity. 4 02 1997 24 04 1997 11 12 2009 Crystallin Lens Cataract Ageing Glycation Protein folding © 1997 S. Karger AG, Basel 1997 Copyright / Drug Dosage / Disclaimer Copyright: All rights reserved. No part of this publication may be translated into other languages...

... of glycation on the structural organization of α-crystallin was evaluated by FPLC analysis of native (pH 6.8 and 8.2) and glycated protein in dissociating conditions. Results suggest that the glycation acts on the protein surface by altering its charge distribution. 11 12 2009 © 1996 S. Karger AG...

...O.K. Argirov; I.I. Kerina Glycation is thought to be a factor in the development of cataract in elderly and diabetic patients. In this report, we describe our initial investigations on the reaction of the dipeptide glycyl-phenylalanine (GF) with different carbonyl compounds as a model of protein...

... aggregation, compared to PLP alone. PLP binds to lens protein amino groups decreasing the sugar binding, but in preventing glycation by PLP attention should be paid to the consequences of its own binding to proteins in diabetes. 11 12 2009 © 1996 S. Karger AG, Basel 1996 Copyright / Drug Dosage...

...Robert Blakytny; John J. Harding We had previously shown that glutathione reductase was inactivated by glycation, and decided to use this model system to see whether bovine α-crystallin could provide protection, and whether its modification had an effect. Other proteins were used as a comparison...

...Martinus A.M. van Boekel; Simone E.A. Hoogakker; John J. Harding; Wilfried W. de Jong We investigated the influence of phosphorylation, glycation, carbamylation and oxidative modification on the capacity of α-crystallin to protect β-crystallins against heat denaturation. Simple modification...

...Mary Cherian; Edathara C. Abraham Human lenses of three different ages were used to study the effect of age and aspirin treatment on glycation of α-, β- and γ-crystallins. Soluble lens proteins were subjected to in vitro glycation with 5 m M [ 14 C]glucose in the presence and absence of 10 m M...

...P. Vidal; J. Cabezas-Cerrato Differential scanning spectroscopy revealed a clear relationship between the degree of in vitro glycation of pig lens crystallins and its optical density at the range of 350-400 nm. This relationship is also shown in bovine serum albumin, used as a standard. In order...

...J.N. Liang The browning reaction (or advanced glycation) of calf lens α-crystallin generates chromophores which absorb light at wavelengths above 300 nm. They display an absorption shoulder above 300 nm, a positive circular dichroism band at 320–340 nm, and a blue fluorescence at 460 nm (Ex = 370...

...Simi Padival; Ram H. Nagaraj Purpose: Advanced glycation end products (AGEs) play an important role in protein modification during cataract formation. Along with sugars, α-dicarbonyl compounds, such as methylglyoxal (MGO), have been implicated in AGE formation. Here we report the effect...

...Johan Bours; Michael H.J. Ahrend; Klaus J. Utikal The aim of the current study was to demonstrate glycation of β L -, β S - and γ-crystallins in the young bovine lens. To establish which of the crystallins are glycated and where they are located in the lens, we carried out microsectioning...