...Takashi Hatano; Masami Kojima In order to develop a more adequate ultraviolet (UV)-induced cataract model, a combination of /-buthionine sulfoximine (BSO) treatment was applied to experimental animals. A single administration of BSO 4 mmol/ kg caused reduced glutathione concentration until...

...(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. Cataract model Crystallin Glutathione Lens Phase separation Rat Selenite Ophthalmic, Research Ophthalmic Res...

...William B. Rathbun Otto Hockwin influenced the early research career in ophthalmic biochemistry of William Rathbun in several ways. These included multiple exposures via symposia to the European research community, providing editorial experience and encouragement to expand fundamental glutathione...

...Bhargavan Chakrapani; Sunita Yedavally; Victor Leverenz; Frank J. Giblin; Venkat N. Ready A sensitive, electrochemical method was employed for the simultaneous measurement of reduced and oxidized glutathione in lens cortex, nucleus and capsule epithelia of rabbit lenses, normal human lenses...

... Glutathione Ophthalmic Res I995:27(suppl 1): 143 149 Keith Green Department of Ophthalmology. Medical College o f Georgia. Augusta. Ga.. USA Free Radicals and Aging of Anterior Segment Tissues of the Eye: A Hypothesis Key Words Aging Lens Cornea Trabecular meshwork Hydrogen peroxide Catalase Glutathione...

... of propylene glycol (1.5 mmol/egg) at 3, 10 and 20 h after HC treatment effectively prevented the HC-induced cataract formation (I: 84%, II: 12%, III: 4%, IV-V: 0%) and repressed the decline of glutathione and the elevation of lipid peroxide in the lens caused by HC. Propylene glycol is metabolized to lactate...

...Lu Wang; Tim T. Lam; Kwok-Wai Lam; Mark O.M. Tso We hypothesize that the differential susceptibility to photic injury among different strains of rat retinas may depend on the levels of phospholipid hydroperoxide glutathione peroxidase (PHGPX) activity, one of the endogenous antioxidant enzymes...

...% of the eyes within 5-6 days. Treatment with naproxen resulted in showing a significant preventive effect. The biochemical parameters like glutathione, malonaldehyde or soluble and insoluble protein contents indicate that selenite causes cataract due to oxidative stress. The observations also suggest...

...A.P. Costarides; M.V. Riley; K. Green The effects of inhibition of both glutathione synthesis and of glutathione reductase and catalase activities have been determined in the regulation of hydrogen peroxide (H 2 O 2 ) in the anterior chamber of pigmented rabbits. Glutathione reductase inhibition...

... Norway rats resulted in gradual, progressive development of zonular opacities. As compared to control animals, the values of soluble protein, soluble glutathione (total of oxidized plus reduced) and activities of glutathione peroxidase and glutathione reductase were decreased in rats fed either...

... and oxidized glutathione (GSH, GSSG) levels in RBC were similar among the three groups. Plasma levels of GSSG were higher in diabetics than in cataractous and control subjects. No differences in plasma content of GSH were noted among the three groups. The activity of the enzyme glucose-6-phosphate...

...Jochen Graw; Wolf Bors; Christa Michel; Peter Reitmeir; Karl-Heinz Summer; Andrea Wulff To determine whether an unbalanced redox state might accompany the development of particular inherited mouse cataracts, the lenticular content of oxidized glutathione (GSSG) and the activity of superoxide...

... of mice with L-buthionine sulfoximine alone resulted in a marked reduction of the glutathione content in the eyes. This deprivation of glutathione was mitigated, to a significant degree, by coadministering γ-glutamylcysteine ethyl ester. In an experiment with rat lens in culture, γ-glutamylcysteine ethyl...

...P.Y. Xie; A. Kanai; A. Nakajima; S. Kitahara; A. Ohtsu; K. Fujii Glutathione and its related enzymes were measured for normal and cataractous human lenses. Glutathione decreased progressively with the development of cataracts. This decrease was more pronounced in the nucleus than in the capsule...

... and the epithelium are apparently uninjured and glucose is available in the incubation medium. When the capsule is removed and glucose is not added, there is a fall of free glutathione and oxidation of water-soluble proteins. Experimental uveitis causes a very early change in conformation of structural lens proteins...

...William B. Rathbun; Magdaline G. Bovis; Ann M. Holleschau Lenses from representative species of eight mammalian orders were assayed for glutathione peroxidase and glutathione reductase activities. Wide variation of glutathione peroxidase activity was noted, the lowest activity being...

...G.N. Rao; B. Sadasivudu; E. Cotlier The cause(s) for decreased reduced glutathione (GSH) content of human senile cataracts were investigated by determination of three enzymes of GSH metabolism: GSH S-transferase, GSH peroxidase and GSH reductase. There are significant decreases in activities of GSH...

...William B. Rathbun The activities of γ-glutamylcysteine synthetase (γ-GCS) and glutathione synthetase, the two enzymes responsible for glutathione synthesis, were determined in adult lenses from representative species of eight mammalian orders. Lenses from Old World higher simians, including man...

...; group C: 4.068 ± 0.538 μmol/lens). Also ascorbic acid levels showed a significant decrease both in lens and in aqueous humor. No differences were noted in the reduced glutathione content in aqueous humor. The fall in nonprotein and protein –SH could be the first event in the well-known biochemical...

...M. Reim; B. Heuvels; H. Cattepoel Glutathione peroxidase activity was estimated in the corneal epithelium, conjunctiva, lens and retina of rabbit and cattle eyes using an enzymatic optical method. The Michaelis constant of the enzyme for hydrogen peroxide was found to be below 1 × 10 ––5 M H 2 O 2...