... for calpains I and II in young rat lenses. An in vitro model of crystallin precipitation was used to assess the ability of calpain I to induce hydrolysis and precipitation of crystallins. We found that incubation of crystallins with purified calpain I was indeed a powerful inducer of crystallin precipitation...

...J. Bours; A.A. El-Layeh; M.H. Emarah; H. Rink Morphologically classified lenses from 100 cataract patients from the Mansoura Eye Hospital (Egypt) were microdissected and the crystallin composition and distribution were analyzed by thin-layer isoelectric focusing (IEF). The IEF profiles...

... the individual crystallin classes, it was established that alpha and beta crystallins were present in extracts from eye lenses from 5-week-old embryos while gamma crystallins could be detected in extracts from embryonic lenses at the beginning of the 6th gestational week. When analyzed by isoelectric focussing...

...P.C. Pereira; J.S. Ramalho; C.J. Faro; M.C. Mota The water-soluble crystallins from normal human lenses (n = 32), cataractous lenses of diabetic patients (n = 9) and cataractous lenses of nondiabetic patients (n = 9) were analyzed with fast-performance liquid chromatography. Six different...

...Mary Cherian; Edathara C. Abraham Human lenses of three different ages were used to study the effect of age and aspirin treatment on glycation of α-, β- and γ-crystallins. Soluble lens proteins were subjected to in vitro glycation with 5 m M [ 14 C]glucose in the presence and absence of 10 m M...

...S. Swamy-Mruthinti; K. Green; E.C. Abraham Glycation has been implicated in cataract formation. Our earlier studies showed that crystallin glycation enhances oxidation and aggregation, whereas MIP26 glycation affects the membrane permeability. Scheimpflug densitometric analysis has been used...

...J.B. Coulter; A.A. Swanson; T.J. Bond; J. Jeter In order to ascertain the in vivo fate of lens proteins, 13-day-old rats were injected i.p. with 3 μCi of 1- 14 C-DL-valine, and the levels of radio-activity were monitored in the α-, β-, and γ-crystallins for approximately 10 months. The pattern...

...H. Maisel; J. Alcala; N. Lieska; N. Rafferty Analysis of the chick lens intracellular matrix by SDS polyacrylamide gel electrophoresis revealed that the cortical urea-soluble protein is characterized by a high content of non-crystallin polypeptides, especially those of molecular weights of 85,000...

...) pseudoexfoliative material was absent. The avidin-biotin complex immunohistochemical method was applied to the pseudoexfoliative material obtained from 15 porcine experimental precataractous lenses and 1 spontaneously cataractous eye and revealed crystallins as a component of the intraocular pseudoexfoliative...