The soluble proteins of human, cat, bovine, dog, sheep, and swine corneas were tested as antigens and analyzed chemically. One of the immunologic tests was leukocate migration inhibition (LMI). The white cells of patients with diverse keratopathies were studied by LMI with human and animal corneal antigens, while the leukocytes of healthy volunteers were used as controls. The results showed considerable similarity among individuals with a corneal disorder and among controls. In the other immunologic experiment, antiserum was raised in rabbits against soluble human corneal protein. The antiserum gave identical precipitation lines with soluble protein from animal corneas. Separation studies of the human and animal soluble corneal proteins yielded a similar chromatographic profile and several analogous bands on acrylamide gel electrophoresis of the chromatographic peaks.

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