A2E-laden ARPE-19 cells were exposed to a blue light to induce cytotoxicity, in order to investigate the protective effects of various tinted ophthalmic lenses against photo-induced cytotoxicity in human retinal pigment epithelial (RPE) cells laden with A2E, known to be among the etiologies of age-related macular degeneration (AMD). Different-colored tinted lenses with varying levels of tint and different filtering characteristics, such as polarized, blue-cut, and photochromatic lenses, were placed over the cells, and the protective efficacies thereof were evaluated by lactate dehydrogenase assay. When tinted lenses were placed over ARPE-19 cells, there were different reductions in cytotoxicity according to the colors and tint levels. The level of protection afforded by brown-tinted lenses was 6.9, 36.1, and 49% with a tint level of 15, 50, and 80%, respectively. For gray-tinted lenses, the protective effect was 16.3, 35, and 43.4% for the corresponding degree of tint, respectively. In the case of blue-tinted lenses, a protective effect of 20% was observed with 80% tinted lenses, but 15 and 50% tinted lenses provided no significant protection. In addition, photochromic lenses showed a protective effect but blue-cut lenses and polarized lenses provided no significant protection. Tinted lenses significantly reduced cytotoxicity in RPE cells irradiated with blue light. The protection was more efficient in lenses with a brown or gray tint than in blue-tinted lenses. Tinted glasses may provide significant protection against potential blue-light-induced photochemical and photo-oxidative damage in RPE cells.

1.
Decanini A, Nordgaard CL, Feng X, Ferrington DA, Olsen TW: Changes in select redox proteins of the retinal pigment epithelium in age-related macular degeneration. Am J Ophthalmol 2007;143:607-615.e602.
2.
Ben-Shabat S, Parish CA, Hashimoto M, Liu J, Nakanishi K, Sparrow JR: Fluorescent pigments of the retinal pigment epithelium and age-related macular degeneration. Bioorg Med Chem Lett 2001;11:1533-1540.
3.
Ferris FL, Fine SL, Hyman L: Age-related macular degeneration and blindness due to neovascular maculopathy. Arch Ophthalmol 1984;102:1640-1642.
4.
Beatty S, Koh H-H, Phil M, Henson D, Boulton M: The role of oxidative stress in the pathogenesis of age-related macular degeneration. Surv Ophthalmol 2000;45:115-134.
5.
Ben-Shabat S, Parish CA, Vollmer HR, Itagaki Y, Fishkin N, Nakanishi K, Sparrow JR: Biosynthetic studies of A2E, a major fluorophore of retinal pigment epithelial lipofuscin. J Biol Chem 2002;277:7183-7190.
6.
Eldred GE, Lasky MR: Retinal age pigments generated by self-assembling lysosomotropic detergents. Nature 1993;36:724-726.
7.
Fishkin NE, Sparrow JR, Allikmets R, Nakanishi K: Isolation and characterization of a retinal pigment epithelial cell fluorophore: an all-trans-retinal dimer conjugate. Proc Natl Acad Sci USA 2005;102:7091-7096.
8.
Kim SR, Jang YP, Jockusch S, Fishkin NE, Turro NJ, Sparrow JR: The all-trans-retinal dimer series of lipofuscin pigments in retinal pigment epithelial cells in a recessive Stargardt disease model. Proc Natl Acad Sci 2007;104:19273-19278.
9.
Schütt F, Davies S, Kopitz J, Holz FG, Boulton ME: Photodamage to human RPE cells by A2-E, a retinoid component of lipofuscin. Invest Ophthalmol Vis Sci 2000;41:2303-2308.
10.
Young RW: Solar radiation and age-related macular degeneration. Surv Ophthalmol 1988;32:252-269.
11.
Van Norren D, Schellekens P: Blue light hazard in rat. Vision Res 1990;30:1517-1520.
12.
Sparrow JR, Miller AS, Zhou J: Blue light-absorbing intraocular lens and retinal pigment epithelium protection in vitro. J Cataract Refract Surg 2004;30:873-878.
13.
Grimm C, Wenzel A, Williams TP, Rol PO, Hafezi F, Reme CE: Rhodopsin-mediated blue-light damage to the rat retina: effect of photoreversal of bleaching. Invest Ophthalmol Vis Sci 2001;42:497-505.
14.
Zhou J, Sparrow JR: Light filtering in a retinal pigment epithelial cell culture model. Optom Vis Sci 2011;88:759-765.
15.
Parish CA, Hashimoto M, Nakanishi K, Dillon J, Sparrow J: Isolation and one-step preparation of A2E and iso-A2E, fluorophores from human retinal pigment epithelium. Proc Natl Acad Sci USA 1998;95:14609-14613.
16.
Jee EH, Kim SR, Jang YP: Rapid purification method for vitamin A-derived aging pigments A2E and iso-A2E using cation exchange resin. J Chromatogr A 2012;1251:232-235.
17.
Sparrow JR, Parish CA, Hashimoto M, Nakanishi K: A2E, a lipofuscin fluorophore, in human retinal pigmented epithelial cells in culture. Invest Ophthalmol Vis Sci 1999;40:2988-2995.
18.
Hattar S, Lucas RJ, Mrosovsky N, Thompson S, Douglas R, Hankins MW, Lem J, Biel M, Hofmann F, Foster RG: Melanopsin and rod-cone photoreceptive systems account for all major accessory visual functions in mice. Nature 2003;424:75-81.
19.
Mainster M, Turner P: Retinal injuries from light: mechanisms, hazards and prevention. Retina 2006;2:1857-1870.
20.
Behar-Cohen F, Martinsons C, Viénot F, Zissis G, Barlier-Salsi A, Cesarini J, Enouf O, Garcia M, Picaud S, Attia D: Light-emitting diodes (LED) for domestic lighting: any risks for the eye? Prog Retin Eye Res 2011;30:239-257.
21.
Sliney DH, Freasier BC: Evaluation of optical radiation hazards. Appl Opt 1973;12:1-24.
22.
Noell WK: Possible mechanisms of photoreceptor damage by light in mammalian eyes. Vision Res 1980;20:1163-1171.
23.
Sparrow JR, Nakanishi K, Parish CA: The lipofuscin fluorophore A2E mediates blue light-induced damage to retinal pigmented epithelial cells. Invest Ophthalmol Vis Sci 2000;41:1981-1989.
24.
Gaillard ER, Zheng L, Merriam JC, Dillon J: Age-related changes in the absorption characteristics of the primate lens. Invest ophthalmol Vis Sci 2000;41:1454-1459.
25.
Sparrow JR, Zhou J, Ben-Shabat S, Vollmer H, Itagaki Y, Nakanishi K: Involvement of oxidative mechanisms in blue-light-induced damage to A2E-laden RPE. Invest Ophthalmol Vis Sci 2002;43:1222-1227.
26.
Lamb LE, Simon JD: A2E: a component of ocular lipofuscin. Photochem Photobiol 2004;79:127-136.
27.
Sparrow JR, Fishkin N, Zhou J, Cai B, Jang YP, Krane S, Itagaki Y, Nakanishi K: A2E, a byproduct of the visual cycle. Vision Res 2003;43:2983-2990.
28.
Sparrow JR, Boulton M: RPE lipofuscin and its role in retinal pathobiology. Exp Eye Res 2005;80:595-606.
29.
Gomes CC, Preto S: Blue light: a blessing or a curse? Procedia Manufacturing 2015;3:4472-4479.
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