Background: Diabetic retinopathy (DR) is the leading cause of blindness among the working-age population. The earliest morphological manifestation of the disease is pericyte loss, as shown by animal models. Aims: The purpose of this study was to evaluate the presence of pericytes in vitreous samples (VS) from diabetic and nondiabetic patients. Methods: VS from 125 patients with and without diabetes were analyzed. Thirty-three of the VS contained blood vessels and were therefore included in further analysis. Pericyte status was evaluated using α-smooth muscle actin and quantified using the following scoring system: total loss (3), >50% loss (2), <50% loss (1), and no loss (0). Results: Of the 33 VS, 29 samples were from patients with diabetes and 4 from nondiabetic patients. Six diabetic cases had a score of 1, 8 diabetic cases had a score of 2, and 15 cases had a score of 3. A positive correlation between glycemia levels and pericyte loss was observed (p = 0.0016; Spearman's r = 0.61). Moreover, all nondiabetic cases had a score of 0 (sensitivity and specificity = 100%). Conclusion: Pericyte loss in VS might be a sensitive and specific marker of DR that correlates with glycemia levels. Furthermore, VS, which are currently discarded, may contain valuable information for diabetic management.

Li C, Balluz LS, Okoro CA, et al: Surveillance of certain health behaviors and conditions among states and selected local areas - Behavioral Risk Factor Surveillance System, United States, 2009. MMWR Surveill Summ 2011;60:1-250.
Aiello LP, Gardner TW, King GL, et al: Diabetic retinopathy. Diabetes Care 1998;21:143-156.
Aiello LP: Diabetic retinopathy and other ocular findings in the Diabetes Control and Complications Trial/Epidemiology of Diabetes Interventions and Complications study. Diabetes Care 2014;37:17-23.
Lamoureux EL, Tai ES, Thumboo J, et al: Impact of diabetic retinopathy on vision-specific function. Ophthalmology 2010;117:757-765.
Hammes HP, Lin J, Renner O, et al: Pericytes and the pathogenesis of diabetic retinopathy. Diabetes 2002;51:3107-3112.
Zoroquiain P, Vilà N, Bravo-Filho V, et al: Histopathological features of vitreous samples in diabetic patients. IOVS 2014;55:4927.
The effect of intensive treatment of diabetes on the development and progression of long-term complications in insulin-dependent diabetes mellitus. The Diabetes Control and Complications Trial Research Group. N Engl J Med 1993;329:977-986.
Scott IU, Flynn HW Jr, Dev S, et al: Endophthalmitis after 25-gauge and 20-gauge pars plana vitrectomy: incidence and outcomes. Retina 2008;28:138-142.
Micieli JA, Micieli A, Schlenker MB, et al: Growth of medical and surgical vitreoretinal subspecialization in Ontario over 12 years. Can J Ophthalmol 2014;49:312-319.
Hellstrom M, Kalén M, Lindahl P, et al: Role of PDGF-B and PDGFR-beta in recruitment of vascular smooth muscle cells and pericytes during embryonic blood vessel formation in the mouse. Development 1999;126:3047-3055.
Fong DS, Aiello LP, Ferris FL 3rd, Klein R: Diabetic retinopathy. Diabetes Care 2004;27:2540-2553.
Ashton N: Vascular basement membrane changes in diabetic retinopathy. Montgomery Lecture, 1973. Br J Ophthalmol 1974;58:344-366.
Genuth S, Alberti KG, Bennett P, et al: Follow-up report on the diagnosis of diabetes mellitus. Diabetes Care 2003;26:3160-3167.
Herman WH, Cohen RM: Racial and ethnic differences in the relationship between HbA1c and blood glucose: implications for the diagnosis of diabetes. J Clin Endocrinol Metab 2012;97:1067-1072.
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