The non-swelling properties of dogfish corneas were studied using histochemical and biochemical methods for estimating acid mucopolysaccharides. Immersion of the corneas in distilled water caused loss of their final weight. This can be attributed to separation of the corneal epithelium, part of the stroma, and by partial ‘solubilization’ of the corneas. The staining properties of the corneal mucopolysaccharides after brief immersion in distilled water were approximately the same as in untreated controls. After longer immersion, staining was diminished due to elution of the ground substance containing acid mucopolysaccharides. The results of hexosamines and uronic acid determination are discussed with respect to the morphological and histochemical findings.

Keywords:
Cornea, Dogfish, Mucopolysaccharides, acid, Histochemistry, Hydration of cornea, Elasmobranch cornea
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© 1973 S. Karger AG, Basel
1973
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