Differential scanning spectroscopy revealed a clear relationship between the degree of in vitro glycation of pig lens crystallins and its optical density at the range of 350-400 nm. This relationship is also shown in bovine serum albumin, used as a standard. In order to study the glycation browning in a large-scale number of samples, various spectroscopic indices have been tested, and the most discriminating became to include the absorbance at 375 nm with a correction of the background absorption at 300 and 500 nm (A3.W index). This index is proposed as a simple method for quantifying advanced products of the Maillard reaction in crystallins and other biological samples.

Keywords:
Glycation, Browning, Advanced glycation end products, Pig lens crystallins, Bovine serum albumin
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© 1989 S. Karger AG, Basel
1989
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