Young (1st decade) and old (7th decade) normal human lenses were exposed to low-level ( < 1 mW/cm2) broadband UV radiation (300–400 nm). UV-induced effects in the whole lens and the extracted lens proteins were monitored by fluorescence spectroscopy. The lens proteins were also subjected to proton and pulse magnetization NMR spectroscopy. These studies provide further evidence that low-level UV radiation exposure enhances nontryptophan fluorescence and may generate additional (longer wavelength) chromophores. The NMR studies suggest that the young gamma crystallin fraction is particularly sensitive to UV radiation compared with the other crystallins.

Keywords:
Ultraviolet radiation, Lens fluorescence, Lens proteins, Proton NMR spectroscopy, Pulse magnetization NMR analyses
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© 1985 S. Karger AG, Basel
1985
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