Introduction: LED cold light sources are utilized nowadays for endoillumination in vitreoretinal surgery. We investigated the effects of an individual mixing of red, green and blue (RGB) light from an LED illumination prototype for chromovitrectomy. Methods and Technique: A novel RGB LED cold light illumination system (Dieter Mann GmbH, Mainaschaff, Germany) contains 3 RGB light sources, allowing for an individual mixing of light, so that the surgeon can adjust and control the color quality and intensity of the applied light during chromovitrectomy. Results: Adding or subtracting the amount of any RGB light will consecutively change the resulting color of the light in the eye. The visualization of the retina or semitransparent naïve structures like the vitreous depends on the illumination. The visualization of epiretinal membranes stained with vital dyes can be enhanced by an individual adjustment of the applied light. Conclusion: Selective subtraction of one RGB light unit may increase the visibility of delicate structures during pars plana vitrectomy.

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