Aim: To investigate the influence of eccentric scanning on retinal nerve fiber layer (RNFL) analysis using Stratus optical coherence tomography (OCT) in normal Koreans when the eccentricity of scanning occurs in 4 different directions. Methods: Thirty-one eyes of 31 persons underwent 1 concentric disk-centered and 4 superiorly, inferiorly, nasally and temporally eccentric RNFL OCT scans. Results: The average RNFL thickness measured by temporally eccentric scanning (115.1 μm) was significantly greater when compared with that found by concentric scanning (107.1 μm; p < 0.0001). The proportions of eyes outside normal limits on the RNFL analysis map using the criterion of ≥1 clock hour abnormal at the <1% level in superiorly (12/31, 38.7%), nasally (16/32, 51.6%) and temporally (12/31, 38.7%) eccentric scans were significantly different in comparison with the concentric (4/31, 12.9%) scan (p < 0.05). Conclusion: An off-centered OCT scan affected RNFL thickness measurement and its interpretation.

1.
Paunescu LA, Schuman JS, Price LL, Stark PC, Beaton S, Ishkawa H, Wollstein G, Fujimoto J: Reproducibility of nerve fiber thickness, macular thickness, and optic nerve head measurement using Stratus OCT. Invest Ophthalmol Vis Sci 2004;45:1716–1724.
2.
Schuman JS, Pedut-Kloizman T, Hertzmark E, Hee MR, Wilkins JR, Coker JG, Puliafito CA, Fujimoto JG, Swanson EA: Reproducibility of nerve fiber layer thickness measurements using optical coherence tomography. Ophthalmology1996;103:1889–1898.
3.
Baumann M, Gentile RC, Liebmann JM, Ritch R: Reproducibility of retinal thickness measurements in normal eyes using optical coherence tomography. Ophthalmic Surg Lasers 1998;29:280–285.
4.
Schuman JS, Hee MR, Puliafito CA, Wong C, Pedut-Kloizman T, Lin CP, Hertzmark E, Izatt JA, Swanson EA, Fujimoto JG: Quantification of nerve fiber layer thickness in normal and glaucomatous eyes using optical coherence tomography. Arch Ophthalmol 1995;113:586–596.
5.
Budenz DL, Chang RT, Huang X, Knighton RW, Tielsch JM: Reproducibility of retinal nerve fiber thickness measurements using the stratus OCT in normal and glaucomatous eyes. Invest Ophthalmol Vis Sci 2005;46:2440–2443.
6.
Budenz DL, Michael A, Chang RT, McSoley J, Katz J: Sensitivity and specificity of the Stratus OCT for perimetric glaucoma. Ophthalmology 2005;112:3–9.
7.
Patella VM: Stratus OCT: establishment of normative reference values for retinal nerve fiber layer thickness measurements. Carl Zeiss Meditec Inc. 2003. http://www.meditec.zeiss.com/C125679E00525939/EmbedTitelIntern/Stratus_OCT_ndb_paper/$File/czm_ndb_paper.pdf (accessed November 15, 2004).
8.
Jeoung JW, Park KH, Kim TW, Khwarg SI, Kim DM: Diagnostic ability of optical coherence tomography with a normative database to detect localized retinal nerve fiber defects. Ophthalmology 2005;112:2157–2163.
9.
El-Ashry M, Appaswamy S, Deokule S, Pagliarini S: The effect of phacoemulsification cataract surgery on the measurement of retinal nerve fiber layer thickness using optical coherence tomography. Curr Eye Res 2006;31:409–413.
10.
Savini G, Zanini M, Barboni P: Influence of pupil size and cataract on retinal nerve fiber layer thickness measurements by Stratus OCT. J Glaucoma 2006;15:336–340.
11.
Gurses-Ozden R, Ishikawa H, Hoh ST, Liebmann JM, Mistlberger A, Greenfield DS, Dou HL, Ritch R: Increasing sampling density improves reproducibility of optical coherence tomography measurements. J Glaucoma 1999;8:238–241.
12.
Bourne RR, Medeiros FA, Bowd C, Jahanbakhsh K, Zangwill LM, Weinreb RN: Comparability of retinal nerve fiber layer thickness measurements of optical coherence tomography instruments. Invest Ophthalmol Vis Sci 2005;46:1280–1285.
13.
Furuichi M, Kashiwagi K, Tsukahara S: Variance between program versions in measuring optic nerve fiber layer thickness using optical coherence tomography. Ophthalmologica 2002;216:409–414.
14.
Stein DM, Wollstein G, Ishikawa H, Hertzmark E, Noecker RJ, Schuman JS: Effect of corneal drying on optical coherence tomography. Ophthalmology 2006;113:985–991.
15.
Wu Z, Vazeen M, Varma R, Chopra V, Walsh AC, Labree LD, Sadda SR: Factors associated with variability in retinal nerve fiber layer thickness measurements obtained by optical coherence tomography. Ophthalmology 2007;114:1505–1512.
16.
Savini G, Zanini M, Carelli V, Sadun AA, Ross-Cisneros FN, Barboni P: Correlation between retinal nerve fiber layer thickness and optic nerve head size: an optical coherence tomography study. Br J Ophthalmol 2005;89:489–491.
17.
Jonas JB, Gusek GC, Naumann GOH: Optic disk, cup and neuroretinal rim size, configuration, and correlations in normal eyes. Invest Ophthalmol Vis Sci 1988;29:1151–1158.
18.
Caprioli J, Miller JM: Measurement of relative nerve fiber layer surface height in glaucoma. Ophthalmology 1989;96:633–641.
19.
Weinreb RN, Shakiba S, Zangwill L: Scanning laser polarimetry to measure the nerve fiber layer of normal and glaucomatous eyes. Am J Ophthalmol 1995;119:627–636.
20.
Tjon-Fo-Sang MJ, de Vries J, Lemij HG: Measurement by nerve fiber analyzer of retinal nerve fiber layer thickness in normal subjects and patients with ocular hypertension. Am J Ophthalmol 1996;122:220–227.
21.
Anton A, Zangwill L, Emdadi A, Weinreb RN: Nerve fiber layer measurements with scanning laser polarimetry in ocular hypertension. Arch Ophthalmol 1997;115:331–334.
22.
Funaki S, Shirakashi M, Abe H: Relation between size of optic disc and thickness of retinal nerve fiber layer in normal subjects. Br J Ophthalmol 1998;82:1242–1245.
23.
Horn FK, Jonas JB, Martus P, Mardin CY, Budde WM: Polarimetric measurement of retinal nerve fiber layer thickness in glaucoma diagnosis. J Glaucoma 1999;8:353–362.
24.
Bowd C, Weinreb RN, Williams JM, Zangwill LM: The retinal nerve fiber layer thickness in ocular hypertensive, normal, and glaucomatous eyes with optical coherence tomography. Arch Ophthalmol 2000;118:22–26.
25.
Varma R, Skaf M, Barron E: Retinal nerve fiber layer thickness in normal human eyes. Ophthalmology 1996;103:2114–2119 (erratum published in Ophthalmology 1997;104:174).
26.
Dichtl A, Jonas JB, Naumann GOH: Retinal nerve fiber layer thickness in human eyes. Graefe’s Arch Clin Exp Ophthalmol 1999;237:474–499.
27.
Takamoto T, Schwartz B: Differences by quadrant of retinal nerve fiber layer thickness in healthy eyes. J Glaucoma 2002;11:359–364.
28.
Budenz DL, Anderson DR, Varma R, Schuman J, Cantor L, Savell J, Greenfield DS, Patella VM, Quigley HA, Tielsch J: Determinants of normal retinal nerve fiber layer thickness measured by Stratus OCT. Ophthalmology 2007;114:1046–1052.
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