Background: To determine the effect of photodynamic therapy (PDT) in chronic central serous chorioretinopathy (CSCR) and to describe fundus autofluorescence (AF) patterns before and after PDT. Methods: Seven eyes of 7 patients with chronic CSCR were included. Patients underwent complete ophthalmic examination and macular optical coherence tomography (OCT) scans were taken. Fluorescein and indocyanine green angiographies and fundus AF images were obtained by Heidelberg Retinal Angiography 2 (HRA2). PDT was performed according to the standard TAP protocol on the site of leakage area and submacular fluid. The mean follow-up period was 5.4 months. Results: Submacular fluid disappeared in all eyes 1 month after PDT and there was no recurrence during the follow-up. Visual acuity increased two or more lines in 5 eyes. Hyperautofluorescence in the area of previous retinal detachment significantly increased in the first month of PDT compared to preoperative findings. After the first month during the follow-up, increased AF in the area of previous retinal elevation returned to the baseline intensity. Conclusion: PDT of CSCR may result with resolution of subretinal fluid and visual improvement. Fundus AF may be an additional tool for monitoring the functional status of retinal pigment epithelium.